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A high-density genetic linkage map of bronze loquat based on SSR and RAPD markers

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Abstract

We constructed a high-density genetic linkage map of bronze loquat (Eriobotrya deflexa) by using a three-way cross of loquat (Eriobotrya japonica) × (loquat × bronze loquat) and simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. The positions of the SSR loci used in this study were previously identified on reference maps of pears (Pyrus spp.) and apples (Malus spp.). The map of bronze loquat (‘Taiwan loquat No. 1’) consisted of 308 loci including 167 SSRs (8 loquat, 57 pear, and 102 apple SSRs), 140 RAPDs, and the loquat canker resistance gene Pse-a on 19 linkage groups covering a genetic distance of 1036 cM. Almost all loquat linkage groups were aligned to the pear consensus map by using at least two pear or apple SSRs, suggesting that positions and linkages of SSR loci were well conserved between loquat and pear and between loquat and apple. The constructed map may be used to determine the location of genes and quantitative trait loci of interest and to analyze genome synteny in the tribe Pyreae, subfamily Spiraeoideae of the family Rosaceae.

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Acknowledgments

We thank Ms. R. Maemoto and S. Anzai for their technical help. This work was partially supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Development of mitigation and adaptation techniques to global warming in the sectors of agriculture, forestry, and fisheries, C-3-2060).

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Author notes

  1. Shinji Fukuda

    Present address: Center for Education and Research in Agricultural Innovation, Saga University, Saga, 849-0903, Japan

  2. Naofumi Hiehata

    Present address: Ken’ou Development Bureau, Nagasaki Prefectural Government, Isahaya, Nagasaki, 854-0071, Japan

Authors and Affiliations

  1. Agriculture and Forestry Technical Development Center, Nagasaki Prefectural Government, Omura, Nagasaki, 856-0021, Japan

    Shinji Fukuda, Keiichiro Ishimoto & Naofumi Hiehata

  2. Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0818, Japan

    Shusei Sato

  3. NARO Institute of Fruit Tree Science, Tsukuba, Ibaraki, 305-8605, Japan

    Shingo Terakami & Toshiya Yamamoto

Authors
  1. Shinji Fukuda
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  2. Keiichiro Ishimoto
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  4. Shingo Terakami
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  5. Naofumi Hiehata
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Correspondence to Toshiya Yamamoto.

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Data archiving statement

A genetic linkage map of bronze loquat will be submitted to the genome database for Rosaceae (www.rosaceae.org) after the final acceptance.

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Communicated by Y. Tsumura

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Fukuda, S., Ishimoto, K., Sato, S. et al. A high-density genetic linkage map of bronze loquat based on SSR and RAPD markers. Tree Genetics & Genomes 12, 80 (2016). https://doi.org/10.1007/s11295-016-1040-9

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  • DOI: https://doi.org/10.1007/s11295-016-1040-9

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