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Worldwide core collections of tea (Camellia sinensis) based on SSR markers

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Abstract

Tea (Camellia sinensis (L.) O. Kuntze) is the world’s most popular beverage crop. However, to date, no core collection has been selected from worldwide germplasm resources on the basis of genotype data. In this study, we analyzed 788 tea germplasm accessions using 23 simple sequence repeat (SSR) markers. Our population structure analysis divided the germplasms into a Japanese group and an exotic group. The latter could be divided into var. sinensis and var. assamica. The genetic diversity was higher in germplasms from China, Taiwan, India, and Sri Lanka than in those from other countries, and low in germplasms from Japan. Using the number of SSR alleles as a measure of genetic diversity, we developed a core collection consisting of 192 accessions and three subcore collections with 96, 48, and 24 accessions. Although the results might be affected by marker-selection bias, the core 192 collection adequately covered the range of variation of the 788 accessions in floral morphology, and the chemical composition of first-flush leaves. These collections will be powerful tools for breeding and genetic research in tea.

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Acknowledgments

We thank R. Ohsawa at University of Tsukuba and N. Aoki at National Agriculture and Food Research Organization for their helpful comments on the manuscript. We also thank Y. Kuramae and M. Iwata at NARO Institute of Vegetable and Tea Science for their technical assistance.

Data archiving statement

We followed standard Tree Genetics and Genomes policy. All genotype data are deposited in the Dryad Repository doi:10.5061/dryad.5jn04.

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Authors and Affiliations

  1. Makurazaki Tea Research Station, NARO Institute of Vegetable and Tea Science, 87, Seto, Makurazaki, Kagoshima, 898-0087, Japan

    Fumiya Taniguchi & Akiko Ogino

  2. Graduate School of Life and Environmental Sciences, University of Tsukuba, 2-1-18, Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

    Fumiya Taniguchi & Junichi Tanaka

  3. Tamagawa University, 6-1-1, Tamagawa-Gakuen, Machida, Tokyo, 194-8610, Japan

    Keiko Kimura & Satoshi Yamaguchi

  4. Kanaya Tea Research Station, NARO Institute of Vegetable and Tea Science, 2769, Shishidoi, Kanaya, Shimada, Shizuoka, 428-8501, Japan

    Tetsuji Saba

  5. NARO Institute of Crop Science, 2-1-18, Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

    Junichi Tanaka

Authors
  1. Fumiya Taniguchi
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  2. Keiko Kimura
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  3. Tetsuji Saba
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  4. Akiko Ogino
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  5. Satoshi Yamaguchi
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  6. Junichi Tanaka
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Corresponding author

Correspondence to Junichi Tanaka.

Additional information

Communicated by Y. Tsumura

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Supplementary Table 1

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Supplementary Fig. 1

DeltaK values and average maximum correlations in the structure result (PPT 159 kb)

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Taniguchi, F., Kimura, K., Saba, T. et al. Worldwide core collections of tea (Camellia sinensis) based on SSR markers. Tree Genetics & Genomes 10, 1555–1565 (2014). https://doi.org/10.1007/s11295-014-0779-0

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  • DOI: https://doi.org/10.1007/s11295-014-0779-0

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