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Hetero-diploid pollen grains that represent self-compatibility are incompatible with non-self receptors in tetraploid Chinese cherry (Prunus pseudocerasus Lindl)

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Abstract

Chinese cherry (Prunus pseudocerasus) has many natural tetraploid species within Prunus. The pollen grains of tetraploid Chinese cherry are hetero-diploid, the two S-haplotypes in the pollen are a combination of two of the four possible S-haplotypes. The abnormal segregation ratios of pollen-S indicate that a few hetero-diploid pollen grains could inactivate self-stylar S-RNase inside the pollen tube and grow better into the self-ovaries than to the others. In this study, three Chinese cherry cultivars, “Daiba” (S 1 S 2 S 5 S 8 ), “Taishanganying” (S 1 S 2 S 4 S 6 ), and “Laiyangduanzhi” (S 1 S 2 S 8 S x ), were used to investigate the inheritance of hetero-diploid pollen-S alleles in non-self receptors. Genetic analysis showed that the distribution of S-haplotypes is unequally expressed in self- and cross-pollinated progenies. The S 2 -haplotype, which is found with lowest frequency in all progeny plants, is a likely lethal mutation. Moreover, the number of individuals with two different S-haplotypes was also unequal in the two cross-pollinated progenies. Notably, the number of individuals with S 1 S 5 and S 1 S 8 genotypes was larger than other genotypes in the cross-pollinated progeny of “Laiyangduanzhi” × “Daiba”, and the number of individuals with S 1 S 4 , S 1 S 6 , and S 4 S 6 genotypes was larger than S 2 -haplotypes in the cross-pollinated progeny of “Laiyangduanzhi” × “Taishanganying”. These results indicate that only a few genotypes of hetero-diploid pollen grains have the capability to grow into the ovaries of “Laiyangduanzhi”. Interestingly, the pollen grains with S 5 S 8 genotypes, which is self-compatible, was incompatible with the styles of “Laiyangduanzhi”, while the pollen grains with S 1 S 8 , S 1 S 6 and S 4 S 6 genotypes, which are self-incompatible, were compatible with the styles of “Laiyangduanzhi”.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31071759 and 31201604) and the National Department Public Benefit Research Foundation (200903044).

Data Archiving Statement

The full-length sequence of P. pseudocerasus S 1 -RNase, S 2 -RNase, S 4 -RNase, S 5 -RNase, S 6 -RNase, S 8 -RNase, SFB 1 , SFB 4 , SFB 5 , and SFB 6 alleles had been submitted in GeneBank with respective accession numbers: HQ913630, FJ543097, FJ543098, HQ913631, FJ543099, HQ913635, HQ913632, HQ913633, EU253964, and HQ913634.

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People’s Republic of China

    Chao Gu, Jun Wu & Shao-Ling Zhang

  2. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, 430074, People’s Republic of China

    Chao Gu

  3. Key Laboratory for Fruit Biotechnology Breeding of Shandong, Pomological Institute of Shandong, Taian, Shandong, 271000, People’s Republic of China

    Qing-Zhong Liu

  4. International Potato Center (CIP), Avenida La Molina 1895, La Molina, Apartado Postal 1558, Lima, Peru

    M. Awais Khan

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  1. Chao Gu
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  2. Qing-Zhong Liu
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  3. M. Awais Khan
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  4. Jun Wu
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  5. Shao-Ling Zhang
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Correspondence to Jun Wu or Shao-Ling Zhang.

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Communicated by E. Dirlewanger

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Table S1

Rates of fruit setting in self- or cross-pollination of four cultivars (DOC 29 kb)

Table S2

Sequences of primers used in this study (DOC 30 kb)

Table S3

Gamete constitutions in the self-progeny of “Laiyangduanzhi” (DOC 39 kb)

Table S4

Gamete constitutions in the progeny of “Laiyangduanzhi” × “Daiba” (DOC 43 kb)

Table S5

Gamete constitutions in the progeny of “Laiyangduanzhi” × “Taishanganying” (DOC 44 kb)

Fig. S1

PCR products of S-RNases amplified with primers Pru-C2 and Pa-C3R from genomic DNA of the parent (“Laiyangduanzhi”) and some of their self-pollinated progeny. M. Ladder marker; L. “Laiyangduanzhi”; 1-45. Forty-five different individuals (DOC 395 kb)

Fig. S2

PCR products of S-RNases amplified with primers Pru-C2 and Pa-C3R from genomic DNA of two parents (“Laiyangduanzhi” and “Daiba”) and some of their cross-pollinated progeny. M. Ladder marker; L. “Laiyangduanzhi”; T. “Daiba”; 1-44. Forty-four different individuals. (DOC 517 kb)

Fig. S3

PCR products of S-RNases amplified with primers Pru-C2 and Pa-C3R from genomic DNA of two parents (“Laiyangduanzhi” and “Taishanganying”) and some of their cross-pollinated progeny. M. Ladder marker; L. “Laiyangduanzhi”; G. “Taishanganying”; 1-44. Forty-four different individuals. (DOC 545 kb)

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Gu, C., Liu, QZ., Khan, M.A. et al. Hetero-diploid pollen grains that represent self-compatibility are incompatible with non-self receptors in tetraploid Chinese cherry (Prunus pseudocerasus Lindl). Tree Genetics & Genomes 10, 619–625 (2014). https://doi.org/10.1007/s11295-014-0708-2

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