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Genome analysis reveals insights of the endophytic Bacillus toyonensis BAC3151 as a potentially novel agent for biocontrol of plant pathogens

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Abstract

Diseases caused by phytopathogenic microorganisms account for enormous losses for agribusiness. Although Bacillus species are recognized as being antimicrobial producers and some may provide benefits to plants, the association between Bacillus toyonensis and plants has not been studied. In this study, the whole-genome sequenced endophytic B. toyonensis BAC3151, which has demonstrated antimicrobial activity and quorum sensing inhibition of phytopathogenic bacteria, was investigated for its potential for the production of compounds for biocontrol of plant pathogens. Four whole-genome sequenced B. toyonensis strains shared 3811 protein-coding DNA sequences (CDSs), while strain-specific CDSs, such as biosynthetic gene clusters of antimicrobials, were associated with specific chromosomal regions and mobile genetic elements of the strains. B. toyonensis strains had a higher frequency of putative bacteriocins gene clusters than that of Bacillus species traditionally used for the production of antimicrobials. In addition, gene clusters potentially involved in the production of novel bacteriocins were found in BAC3151, as well as biosynthetic genes of several other compounds, including non-ribosomal peptides, N-acyl homoserine lactonase and chitinases, revealing a genetic repertoire for antimicrobial synthesis greater than that of other Bacillus strains that have demonstrated effective activity against phytopathogens. This study showed for the first time that B. toyonensis has potential to produce various antimicrobials, and the analyses performed indicated that the endophytic strain BAC3151 can be useful for the development of new strategies to control microbial diseases in plants that are responsible for large damages in agricultural crops.

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Acknowledgements

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Av. P. H. Rolfs, s/n, 36570-900, Viçosa, MG, Brazil

    Ralf Lopes, Hilário C. Mantovani & Marisa Vieira de Queiroz

  2. Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, 05508-000, Butantã, São Paulo, SP, Brazil

    Louise Cerdeira

  3. Grupo Informática de Biossistemas e Genômica, Centro de Pesquisas René Rachou - Fiocruz Minas, Av. Augusto de Lima, 1715, 30190-002, Barro Preto, Belo Horizonte, MG, Brazil

    Grace S. Tavares, Jeronimo C. Ruiz & Elvira C. A. Horácio

  4. Programa de Pós-graduação em Biologia Computacional e Sistemas, Instituto Oswaldo Cruz - Fiocruz Rio, Av. Brasil, 4365, Pavilhão Arthur Neiva, 21040-360, Manguinhos, Rio de Janeiro, RJ, Brazil

    Grace S. Tavares, Jeronimo C. Ruiz & Elvira C. A. Horácio

  5. Institute for Bioinformatics and Systems Biology, Justus-Liebig-University Giessen, Heinrich Buff Ring 58, 35392, Giessen, Hesse, Germany

    Jochen Blom

Authors
  1. Ralf Lopes
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  2. Louise Cerdeira
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  3. Grace S. Tavares
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  4. Jeronimo C. Ruiz
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  5. Jochen Blom
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  6. Elvira C. A. Horácio
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  7. Hilário C. Mantovani
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  8. Marisa Vieira de Queiroz
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Corresponding author

Correspondence to Marisa Vieira de Queiroz.

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Lopes, R., Cerdeira, L., Tavares, G.S. et al. Genome analysis reveals insights of the endophytic Bacillus toyonensis BAC3151 as a potentially novel agent for biocontrol of plant pathogens. World J Microbiol Biotechnol 33, 185 (2017). https://doi.org/10.1007/s11274-017-2347-x

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  • DOI: https://doi.org/10.1007/s11274-017-2347-x

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