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Gluconic acid production by gad mutant of Klebsiella pneumoniae

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Abstract

Klebsiella pneumoniae produces many economically important chemicals. Using glucose as a carbon source, the main metabolic product in K. pneumoniae is 2,3-butanediol. Gluconic acid is an intermediate of the glucose oxidation pathway. In the current study, a metabolic engineering strategy was used to develop a gluconic acid-producing K. pneumoniae strain. Deletion of gad, resulting in loss of gluconate dehydrogenase activity, led to the accumulation of gluconic acid in the culture broth. Gluconic acid accumulation by K. pneumoniae Δgad was an acid-dependent aerobic process, with accumulation observed at pH 5.5 or lower, and at higher levels of oxygen supplementation. Under all other conditions tested, 2,3-butanediol was the main metabolic product of the process. In fed batch fermentation, a final concentration of 422 g/L gluconic acid was produced by K. pneumoniae Δgad, and the conversion ratio of glucose to gluconic acid reached 1 g/g. The K. pneumoniae Δgad described in this study is the first genetically modified strain used for gluconic acid production, and this optimized method for gluconic acid production may have important industrial applications.

Graphical Abstract

Gluconic acid is an intermediate of this glucose oxidation pathway. Deletion of gad, resulting in loss of gluconate dehydrogenase activity, led to the accumulation of gluconic acid in the culture broth. In fed batch fermentation, a final concentration of 422 g/L gluconic acid was produced by the K. pneumoniae Δgad strain, and the conversion ratio of glucose to gluconic acid reached 1 g/g.

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Acknowledgments

This work was supported by a Grant from the KRIBB Research Initiative Program (KGM2211531) and National Natural Science Foundation of China (Grant Nos. 20906076, 21576279).

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Authors and Affiliations

  1. Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People’s Republic of China

    Dexin Wang, Chenhong Wang, Dong Wei, Jiping Shi, Biao Jiang & Jian Hao

  2. Department of Biological Engineering, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, People’s Republic of China

    Dexin Wang, Chenhong Wang & Zengsheng Han

  3. Biorefinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea

    Dexin Wang & Chul Ho Kim

  4. School of Life Science and Technology, ShanghaiTech University, Shanghai, People’s Republic of China

    Jiping Shi

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  1. Dexin Wang
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  2. Chenhong Wang
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  3. Dong Wei
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  4. Jiping Shi
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  5. Chul Ho Kim
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  6. Biao Jiang
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  7. Zengsheng Han
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  8. Jian Hao
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Correspondence to Zengsheng Han or Jian Hao.

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Wang, D., Wang, C., Wei, D. et al. Gluconic acid production by gad mutant of Klebsiella pneumoniae . World J Microbiol Biotechnol 32, 132 (2016). https://doi.org/10.1007/s11274-016-2080-x

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  • DOI: https://doi.org/10.1007/s11274-016-2080-x

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