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Physiological states and energetic adaptation during growth of Pseudomonas putida mt-2 on glucose

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Abstract

Kinetic study of growth of Pseudomonas putida mt-2 was investigated in batch culture under aerobic conditions, on glucose as initial carbon and energy source. Cell growth was continuous and three phases were found regarding accumulation of intermediates: (1) glucose was largely converted to gluconate and 2-ketogluconate, (2) then gluconate was converted to 2-ketogluconate and (3) the latter was consumed after gluconate depletion. Examination of growth kinetics and yields showed that glucose flux was mainly oriented to oxidation reduction in the periplasm and less towards biosynthesis. Values of respiratory quotient and of CO2/biomass and O2/biomass yields were characteristic of each phase. Main enzymatic activities involved in the use of these substrates were always detected meaning that concomitant assimilation is possible. However the levels of these activities varied during growth. Membrane conversions seem to have a significant energetic contribution explaining the higher specific growth rate obtained in glucose phase compared to gluconate and 2-ketogluconate ones. This is also noticeable through the evolution of the yields \( Y_{{\text{O}}_2/{\text{X}}} \) and \( Y_{{\text{CO}}_2 /{\text{X}}} \). Although the three convergent pathways are operational and can be genetically controlled, the progression of the culture in successive phases highlights an overall level of regulation in response to the energetic needs.

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Abbreviations

μ :

Specific growth rate (h−1)

CPR:

CO2 production rate (mmole l−1 h−1)

Gn:

Gluconate

KGn:

2-ketogluconate

net S:

Net assimilated phase substrate not used in membrane oxidations (C-mole)

OUR:

Oxygen uptake rate (mmole l−1 h−1)

\( q_{{\text{CO}}_2 } \) :

Specific CO2 production rate (mmole g−1 h−1)

\( q_{{\text{O}}_2 } \) :

Specific oxygen uptake rate (mmole g−1 h−1)

RQ:

Respiratory quotient (CPR/OUR)

S:

Growth phase substrate (i.e. glucose, gluconate or 2-ketogluconate)

\( Y_{{\text{CO}}_2 /{\text{S}}} \) :

Yield, in percent of C-mole of accumulated CO2 per C-mole of consumed substrate

\( Y_{{\text{CO}}_2 /{\text{X}}} \) :

Molar yield of produced CO2 to formed biomass (mole/mole)

Y Gn/S :

Yield, in percent of C-mole of accumulated gluconate per C-mole of consumed substrate

Y KGn/S :

Yield, in percent of C-mole of accumulated 2-ketogluconate per C-mole of consumed substrate

\( Y_{{\text{O}}_2 /{\text{X}}} \) :

Molar yield of consumed oxygen to formed biomass (mole/mole)

Y X/net S :

Net conversion yield, in percent of C-mole of formed biomass per C-mole of net assimilated phase substrate

Y X/S :

Yield, in percent of C-mole of formed biomass per C-mole of utilized substrate

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Acknowledgments

We thank Margaret Lemarié and Benoit Basset for their technical assistance.

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Author notes

  1. Ridha Mosrati

    Present address: Département Génie chimique–Génie des Procédés, IUT de Caen, 2, Boulevard du Maréchal Juin, Fr-14 032, Caen cedex, France

Authors and Affiliations

  1. Equipe de Recherche en Physico-Chimie et Biotechnologie (ERPCB-EA3914) IUT-UFR Sciences, Université de Caen Basse-Normandie, 2, Boulevard du Maréchal Juin, Fr-14 032, Caen cedex, France

    Leith Latrach Tlemçani, David Corroler, Daniel Barillier & Ridha Mosrati

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  1. Leith Latrach Tlemçani
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  2. David Corroler
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Correspondence to Ridha Mosrati.

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Communicated by Stuart Ferguson.

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Latrach Tlemçani, L., Corroler, D., Barillier, D. et al. Physiological states and energetic adaptation during growth of Pseudomonas putida mt-2 on glucose. Arch Microbiol 190, 141–150 (2008). https://doi.org/10.1007/s00203-008-0380-8

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  • DOI: https://doi.org/10.1007/s00203-008-0380-8

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