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Expression of glycoproteins bearing complex human-like glycans with galactose terminal in Hansenula polymorpha

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Abstract

Glycoproteins derived from Hansenula polymorpha can not be used for therapeutic purposes due to their high-mannose type asparagine-linked (N-linked) glycans, which result in immune reactions and poor pharmacokinetic behaviors in human body. Previously, we reported that the trimannosyl core N-linked glycans (Man3GlcNAc2) intermediate can be generated in endoplasmic reticulum in HpALG3 and HpALG11 double-mutant H. polymorpha. Here, we describe the further modification of the glycosylation pathway in this double-defect strain to express glycoproteins with complex human-like glycans. After eliminating the impact of HpOCH1, three glycosyltransferases were introduced into this triple-mutant strain. When human β-1,2-N-acetylglucosaminyltransferase I (hGnTI) was efficiently targeted in early Golgi, more than 95 % glycans attached to the glycoproteins were added one N-acetylglucosamine (GlcNAc). With subsequently introduction of rat β-1,2-N-acetylglucosaminyltransferase II (rGnTII) and human β-1,4-galactosyltransferase I (hGalTI), several glycoengineered strains can produce glycoproteins bearing glycans with terminal N-acetylglucosamine or galactose. The expression of glycoproteins with glycan Gal2GlcNAc2Man3GlcNAc2 represents a significant step toward the ability to express fully humanized glycoproteins in H. polymorpha. Furthermore, several shake-flask and bioreactor fermentation experiments indicated that, although the cells do display a reduction in growth rate, the glycoengineered strains are still suitable for high-density fermentation.

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Acknowledgments

We gratefully acknowledge the MALDI-TOF assistance provided by Dr. Yuan-ming Luo (Institute of Microbiology, Chinese Academy of Sciences, Beijing, China).

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The authors report no potential conflicts of interest in this work.

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Authors and Affiliations

  1. Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, 100101, China

    Hui Wang, Hao-lei Song, Qian Wang & Bing-sheng Qiu

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  1. Hui Wang
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  2. Hao-lei Song
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  3. Qian Wang
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  4. Bing-sheng Qiu
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Correspondence to Bing-sheng Qiu.

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Wang, H., Song, Hl., Wang, Q. et al. Expression of glycoproteins bearing complex human-like glycans with galactose terminal in Hansenula polymorpha . World J Microbiol Biotechnol 29, 447–458 (2013). https://doi.org/10.1007/s11274-012-1197-9

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  • DOI: https://doi.org/10.1007/s11274-012-1197-9

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