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Diversity of culturable root-associated/endophytic bacteria and their chitinolytic and aflatoxin inhibition activity of peanut plant in China

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Abstract

A total of 72 isolates of root-associated/endophytic (RAE) bacteria were isolated from peanut plants grown in the main producing areas of 6 provinces in China. The 16S rRNA gene sequences of these isolates were determined and phylogenetic analyses revealed that 72 isolates belonged to the classes Bacilli (49 isolates) and Gammaproteobacteria (23 isolates). The majority of RAE bacteria in Bacilli belonged to 2 genera, Bacillus and Lysinibacillus (48 and 1) while those in Gammaproteobacteria belonged to the genera Enterobacter, Serratia, Stenotrophomonas, and Pseudomonas (7, 11, 3 and 2 isolates, respectively). This is the first report of Lysinibacillus xylanilyticus isolate as biocontrol agent against AFs. All of the selected RAE bacteria showed inhibitory activities against Aspergillus parasiticus (A. parasiticus) growth and/or aflatoxins (AFs) production by visual agar plate assay and tip culture method. Most of the RAE bacteria strains (96 % strains) were determined to have decreased mycelia growth or AFs production levels by >50 % (p < 0.05). Bacterial isolates were further characterized for chitinolytic activity and 22 strains (30 % strains) of identified RAE bacteria degraded colloidal chitin on the chitin medium plate. Ten selected chitinolytic RAE bacteria were tested for antifungal activity on peanuts and most of them significantly decreased mycelial growth and AFs production levels by >90 %. These results showed a wide distribution of biological control bacteria against AFs in Chinese peanut main producing areas and the selected RAE bacteria could potentially be utilized for the biocontrol of toxicogenic fungi.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30571244 and No. 30870003) and Project(HIT-NSRIF200806)supported by Natural Scientific Research Innovation Foundation in Harbin Institute of Technology.

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Authors and Affiliations

  1. School of Municipal & Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China

    Kai Wang & Pei-sheng Yan

  2. School of Marine Science and Technology, Harbin Institute of Technology, 264209, Weihai, China

    Kai Wang, Pei-sheng Yan, Qing-long Ding, Qin-xi Wu, Zhong-bo Wang & Jie Peng

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  1. Kai Wang
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  2. Pei-sheng Yan
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Correspondence to Kai Wang or Pei-sheng Yan.

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Wang, K., Yan, Ps., Ding, Ql. et al. Diversity of culturable root-associated/endophytic bacteria and their chitinolytic and aflatoxin inhibition activity of peanut plant in China. World J Microbiol Biotechnol 29, 1–10 (2013). https://doi.org/10.1007/s11274-012-1135-x

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