Abstract
Environmental control plots adjusted to late season drought and elevated soil temperatures where inoculated at peanut planting with low and high levels of conidia, sclerotia, and mycelium from a brown conidial mutant ofAspergillus parasiticus. Percentage infection of peanut seeds from undamaged pods was greatest for the subplot containing the high sclerotial inoculum (15/cm2 soil surface). Sclerotia did not germinate sporogenically and may have invaded seeds through mycelium. In contrast, the mycelial inoculum (colonized peanut seed particles) released large numbers of conidia into soil. Soil conidial populations of brownA. parasiticus from treatments with conidia and mycelium were positively correlated with the incidence of seed infection in undamaged pods. The ratio ofA. flavus to wild-typeA. parasiticus in soil shifted from 7:3 to 1:1 in the uninoculated subplot after instigation of drought, whereas in all subplots treated with brownA. parasiticus, the ratio of the two species became approximately 8:2. Despite high levels of brownA. parasiticus populations in soil, nativeA. flavus often dominated peanut seeds, suggesting that it is a more aggressive species. Sclerotia of wild-typeA. parasiticus formed infrequently on preharvest peanut seeds from insect-damaged pods.
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References
Angle JS, Dunn KA, Wagner GH. Effect of cultural practices on the soil population ofAspergillus flavus andAspergillus parasiticus. Soil Sci Soc Am J 1982; 46: 301–4.
Lillehoj EB, McMillian WW, Guthrie WD, Barry D. Aflatoxin-producing fungi in preharvest corn: Inoculum source in insects and soils. J Environ Qual 1980; 9: 691–94.
Manabe M, Tsuruta O, Tanaka K, Matsuura S. Distribution of aflatoxin-producing fungi in soil in Japan. Trans Mycol Soc Japan 1976; 17: 436–44.
Hill RA, Blankenship PD, Cole RJ, Sanders TH. Effects of soil moisture and temperature on preharvest invasion of peanuts by theAspergillus flavus group and subsequent aflatoxin development. Appl Environ Microbiol 1983; 45: 628–33.
Pitt JI, Hocking AD. Fungi and food spoilage. Sydney: Academic Press, 1985: 293.
Kurtzman CP, Smiley MJ, Robnett CJ, Wicklow DT. DNA relatedness among wild and domesticated species in theAspergillus flavus group. Mycologia 1986; 78: 955–59.
Klich MA, Pitt JI. Differentiation ofAspergillus flavus fromA. parasiticus and other closely related species. Trans Br Mycol Soc 1988; 91: 99–108.
Raper KB, Fennell DI. The genusAspergillus. Baltimore: Williams and Wilkins, 1965: 357–404.
Dorner JW, Cole RJ, Diener UL. The relationship ofAspergillus flavus andAspergillus parasiticus with reference to production of aflatoxins and cyclopiazonic acid. Mycopathologia 1984; 87: 13–15.
Gallagher RT, Richard JL, Stahr HM, Cole RJ. Cyclopiazonic acid production by aflatoxigenic and non-aflatoxigenic strains ofAspergillus flavus. Mycopathologia 1978; 66: 31–36.
Blankenship PD, Cole RJ, Sanders TH, Hill RA. Effect of geocarposphere temperature on pre-harvest colonization of drought-stressed peanuts byAspergillus flavus and subsequent aflatoxin contamination. Mycopathologia 1984; 85: 69–74.
Cole RJ, Sanders TH, Hill RA, Blankenship PD. Mean geocarposphere temperatures that induce preharvest aflatoxin contamination of peanuts under drought stress. Mycopathologia 1985; 91: 41–46.
Dorner JW, Cole RJ, Sanders TH, Blankenship PD. Interrelationship of kernel water activity, soil temperature, maturity, and phytoalexin production in preharvest aflatoxin contamination of drought-stressed peanuts. Mycopathologia 1989; 105: 117–28.
Sanders TH, Cole RJ, Blankenship PD, Hill RA. Relation of environmental stress duration toAspergillus flavus invasion and aflatoxin production in preharvest peanuts. Peanut Sci 1985; 12: 90–93.
Griffin GJ, Garren KH. Population levels ofAspergillus flavus and theA. niger group in Virginia peanut field soils. Phytopathology 1974; 64: 322–25.
Bell DK, Crawford JL. A Botran-amended medium for isolatingAspergillus flavus from peanuts and soil. Phytopathology 1967; 57: 939–41.
Griffin GJ, Ford RH, Garren KH. Relation ofAspergillus flavus colony growth on three selective media to recovery from naturally infested soil. Phytopathology 1975; 65: 704–7.
Griffin GJ, Conidial germination and population ofAspergillus flavus in the geocarposphere of peanut. Phytopathology 1972; 62: 1387–91.
Griffin GJ, Garren KH. Colonization of rye green manure and peanut fruit debris byAspergillus flavus andAspergillus niger group in field soils. Appl Environ Microbiol 1976; 32: 28–32.
Wicklow DT. Survival ofAspergillus flavus sclerotia in soil. Trans Br Mycol Soc 1987; 89: 131–34.
Wicklow DT. Horn BW, Burg WR, Cole RJ. Sclerotium dispersal ofAspergillus flavus andEupenicillium ochrosalmoneum from maize during harvest. Trans Br Mycol Soc 1984; 83: 299–303.
Wicklow DT, Donahue JE. Sporogenic germination of sclerotia inAspergillus flavus andA. parasiticus. Trans Br Mycol Soc 1984; 82: 621–24.
Blankenship PD, Cole RJ, Sanders TH, Hill RA. Environmental control plot facility with manipulable soil temperature. Oléagineux 1983; 38: 615–18.
Delaplane KS, ed. Georgia pest control handbook; special bulletin 28. Athens: University of Georgia College of Agriculture, 1991: 66–74.
Williams EJ, Monroe GE. Impact blasters for peanut pod maturity determination. Trans Am Soc Agric Eng 1986; 29: 263–66 & 275.
Williams EJ, Drexler JS. A non-destructive method for determining peanut pod maturity. Peanut Sci 1981; 8: 134–41.
Lee LS, Bennett JW, Goldblatt LA, Lundin RE. Norsolorinic acid from a mutant strain ofAspergillus parasiticus. J Am Oil Chem Soc 1971; 48: 93–94.
Bennett JW, Goldblatt LA. The isolation of mutants ofAspergillus flavus andA. parasiticus with altered aflatoxin producing ability. Sabouraudia 1973; 11: 235–41.
Cole RJ, Hill RA, Blankenship PD, Sanders TH. Color mutants ofAspergillus flavus andAspergillus parasiticus in a study of preharvest invasion of peanuts. Appl Environ Microbiol 1986; 52: 1128–31.
Lynch RE, Wilson DM. Enhanced infection of peanut,Arachis hypogaea L., seeds withAspergillus flavus group fungi due to scarification of peanut pods by the lesser cornstalk borer,Elasmopalpus lignosellus (Zeller). Peanut Sci 1991; 18: 110–16.
King AD, Jr, Hocking AD, Pitt JI. Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Appl Environ Microbiol 1979; 37: 959–64.
Dorner JW, Cole RJ. Rapid determination of aflatoxins in raw peanuts by liquid chromatography with postcolumn iodination and modified minicolumn cleanup. J Assoc Off Anal Chem 1988; 71: 43–47.
Kok, W, Van Neer CH, Traag WA, Tuinstra LGM. Determination of aflatoxins in cattle feed by liquid chromatography and post-column derivatization with electrochemically generated bromine. J Chromatogr 1986; 367: 231–36.
Official methods of analysis, 15th ed. Arlington, VA: AOAC, 1990: 1186–87.
Stack JP, Pettit RE. Germination ofAspergillus flavus sclerotia in soil. Phytopathology 1984; 74: 799.
Stack JP, Pettit RE. Colonization of organic matter substrates in soil byAspergillus flavus. Proc Am Peanut Res Educ Soc 1984; 16: 45.
Porter DM, Smith DH, Rodríguez-Kábana R, eds. Compendium of peanut diseases. St. Paul, MN: American Phytopathological Society, 1984: 15–38.
Wicklow DT, Horn BW.Aspergillus flavus sclerotia form in wound-inoculated preharvest corn. Mycologia 1984; 76: 503–5.
Joffe AZ. The mycoflora of groundnut rhizosphere, soil and geocarposphere on light, medium and heavy soils and its relations toAspergillus flavus. Mycopathol Mycol Appl 1969; 37: 150–60.
Coley-Smith JR, Cooke RC. Survival and germination of fungal sclerotia. Ann Rev Phytopathol 1971; 9: 65–92.
Sanders TH, Hill RA, Cole RJ, Blankenship PD. Effect of drought on occurrence ofAspergillus flavus in maturing peanuts. J Am Oil Chem Soc 1981; 58: 966A-70A.
Pitt JI, Dyer SK, McCammon S. Systemic invasion of developing peanut plants byAspergillus flavus. Letters Appl Microbiol 1991; 13: 16–20.
Hill RA, Wilson DM, McMillian WW, Widstrom NW, Cole RJ, Sanders TH, Blankenship PD. Ecology of theAspergillus flavus group and aflatoxin formation in maize and groundnut. In: Lacey J, ed. Trichothecenes and other mycotoxins. Chichester, UK: John Wiley and Sons, 1985: 79–95.
Calvert OH, Lillehoj EB, Kwolek WF, Zuber MS. Aflatoxin B1 and G1 production in developingZea mays kernels from mixed inocula ofAspergillus flavus andA. parasiticus. Phytopathology 1978; 68: 501–6.
Joffe AZ. Aflatoxin produced by 1,626 isolates ofAspergillus flavus from groundnut kernels and soils in Israel. Nature 1969; 221: 492.
Dorner JW, Cole RJ, Blankenship PD. Use of a biocompetitive agent to control preharvest aflatoxin in drought stressed peanuts. J Food Prot 1992; 55: 888–92.
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Horn, B.W., Dorner, J.W., Greene, R.L. et al. Effect ofAspergillus parasiticus soil inoculum on invasion of peanut seeds. Mycopathologia 125, 179–191 (1994). https://doi.org/10.1007/BF01146524
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DOI: https://doi.org/10.1007/BF01146524