Abstract
The aims of this study were to select bacterial isolates from the non-rhizophere of maize soil and to examine their antagonistic activity against Aspergillus section Flavi strains. The first selection was made through ecophysiological responses of bacterial isolates to water activity (a w) and temperature stress. Subsequently, an Index of Dominance test (I D), ecological similarity and inhibition of the lag phase prior to growth, growth rate and aflatoxin B1 accumulation were used as criteria. From the first assay nine bacterial strains were selected. They grew well at 25 and 30 °C, with growth optima between 0.982 and 0.955 a W using 48 h of incubation. There was ecological similarity between the bacterial strains Bacillus subtilis (RCB 3, RCB 6), Pseudomonas solanacearum RCB 5, Amphibacillus xylanus RCB 27 and aflatoxigenic Aspergillus section Flavi strains at 0.982 at 25 °C. The predominant interaction between all selected bacteria and fungi in dual culture was mutual intermingling at 0.982. Mutual inhibition on contact and mutual inhibition at a distance was observed at 0.955 a w, between only four bacteria and some Aspergillus strains. Bacillus subtilis RCB 55 showed antifungal activity against Aspergillus section Flavi strains. Amphibacillus xylanus RCB 27, B.␣subtilis RCB 90 and Sporolactobacillus inulinus RCB 196 increased the lag phase prior to growth and decreased the growth rate of Aspergillus section Flavi strains. Bacillus subtilis strains (RCB 6, RCB 55, RCB 90) and P. solanacearum RCB 110 inhibited aflatoxin accumulation. Bacillus subtilis RCB 90 completely inhibited aflatoxin B1 accumulation at 0.982 a W. These results show that the bacterial strains selected have potential for controlling Aspergillus section Flavi over a wide range of relevant environmental conditions in the stored maize ecosystem.
Similar content being viewed by others
References
RL Brown PJ Cotty TE Cleveland (1991) ArticleTitleReduction in aflatoxin content of maize by atoxigenic strains of Aspergillus flavus Journal of Food Protection 54 223–226
InstitutionalAuthorNameCAST (1989) Mycotoxins, Economic and Healt Risks, Task Force Rep. 116 Council for Agricultural Science and Technology Ames, IA
S Chulze C Bertinetti A Dalcero M Etcheverry C Farnochi A Torres I Rizzo E Varsavsky (1989) ArticleTitleIncidence of aflatoxin zearalenone and deoxynivalenol on corn in Argentina Mycotoxin Research 5 9–12 Occurrence Handle1:CAS:528:DyaK3cXksFCjs7c%3D
G Clevstrom H Ljungreen (1985) ArticleTitleAflatoxin formation and the dual phenomenon in Aspergillus flavus Link Mycopathologia 92 129–139 Occurrence Handle3937056 Occurrence Handle1:STN:280:BimC3MfmtFE%3D
RJ Cole PJ Cotty (1990) Biocontrol of aflatoxin production by using biocompetitive agents, JF Robens (Eds) A Perspective on Aflatoxin in Field Crops and Animal Food Products in the United States ARS-83 Agricultural Research Service U.S. Department of Agriculture Washington, DC 62–66
PJ Cotty D Bhatnagar (1994) ArticleTitleVariability among atoxigenic Aspergillus flavus strains in ability to prevent aflatoxin contamination and production of aflatoxin biosynthetic pathway enzymes Applied and Environmental Microbiology 60 IssueID7 2248–2251 Occurrence Handle8074506 Occurrence Handle1:CAS:528:DyaK2cXkslyitLk%3D
H Dallyn A Fox (1980) Spoilage of material of reduced water activity by xerophilic fungi GH Gould EL Corry (Eds) Society of Applied Bacteriology Technical Series Academic Press London 129–139
UL Diener RJ Cole TH Sanders GA Payne LS Lee MA Klich (1987) ArticleTitleEpidemiology of aflatoxin formation by Aspergillus flavus Annual Review of Phytopathology 25 249–270 Occurrence Handle1:CAS:528:DyaL2sXmtlaltbs%3D
JW Dorner RJ Cole (2002) ArticleTitleEffect of application of nontoxigenic strains of Aspergillus flavus and A. parasiticus on subsequent aflatoxin contamination of peanuts in storage Journal of Stored Products Research 38 329–339 Occurrence Handle10.1016/S0022-474X(01)00035-2 Occurrence Handle1:CAS:528:DC%2BD38XktVChtbo%3D
JW Dorner RJ Cole PD Blankenship (1998) ArticleTitleEffect of inoculum rate of biological control agents on preharvest aflatoxin contamination of peanuts Biological Coantrol 12 171–176
R Geisen (1996) ArticleTitleMultiplex Plymerase Chain Reaction for the Detection of Potential Aflatoxin and Sterigmatocystin Producing Fungi, System Applied Microbiology 19 388–392 Occurrence Handle1:CAS:528:DyaK2sXhsVCksg%3D%3D
H Gourama LB Bullerman (1995) ArticleTitle Aspergillus flavus and Aspergillus parasiticus: Aflatoxicogenic fungi of concern in foods and feeds: A review Journal of Food Protection 58 1395–1404 Occurrence Handle1:CAS:528:DyaK28XotVCgtQ%3D%3D
N Gqaleni JE Smith Gettinby (1996) ArticleTitleThe production of cyclopiazonic acid by Penicillium commune and cyclopiazonic acid and aflatoxin by Aspergillus flavus as affected by water activity and temperature on maize grains Mycopathologia 136 103–108 Occurrence Handle1:CAS:528:DyaK2sXksVejtLo%3D
Holt JG, Krieg N, Sneath PHA, Staley JT and Williams ST (1994) In: Williams, Eilkins, (eds) Bergey’s Manual of Determinative Bacteriology, 9th edn. Baltimore, USA
N Kimura S Hirano (1988) ArticleTitleInhibitory strain of Bacillus subtilis for grown and aflatoxin-production of aflatoxicogenic fungi Agricultural Biology and Chemistry 52 1173–1179 Occurrence Handle1:CAS:528:DyaL1cXksFWls7k%3D
CP Kurtzman BW Horn CW Hesseltine (1987) ArticleTitle Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii Antonie van Leeuwenhoek 53 147–158 Occurrence Handle10.1007/BF00393843 Occurrence Handle3116923 Occurrence Handle1:STN:280:BieD383msVU%3D
J Lacey N Magan ((1991)) Mycotoxins, fungi and quality in drying and storage J Chelkowski (Eds) Cereal Grain Elsevier Science Publisher BV Amsterdam
N Magan J Lacey (1984) ArticleTitleEffect of water activity, temperature and substrate on interactions between field and storage fungi Transactions of British Mycological Society 82 83–93
S Marín V Sanchis N Magan (1995) ArticleTitleWater activity, temperature and pH effects on growth of Fusarium moniliforme and Fusarium proliferatum isolates from maize Canadian Journal of Microbiology 41 1063–1070 Occurrence Handle8542550
S Marín V Sanchis AJ Ramos I Vinas N Magan (1998) ArticleTitleEnvironmental factors, in vitro interactions, and niche overlap between Fusarium moniliforme, F. proliferatum, and F. graminearum, Aspergillus and Penicillium species from maize grain Mycological Research 102 831–837
C Munimbazi LB Bullerman (1998) ArticleTitleInhibition of aflatoxin production of Aspergillus parasiticus NRRL 2999 by Bacillus pumilus Mycophatologia 140 163–169 Occurrence Handle1:CAS:528:DyaK1cXls1Cnsbk%3D
EB Nelson WL Chad JM Norton GT Nash GE Harman (1986) ArticleTitleAttachment of Enterobacter cloacae to hyphae of Pythium ultimum: Possible role in the biological control of Pythium pre-emergence damping off Phytopathology 76 327–335
A Nesci M Etcheverry (2002) ArticleTitle Aspergillus section Flavi populations from field maize in Argentina Letters in Applied Microbiology 34 1–6 Occurrence Handle10.1046/j.1472-765X.2002.01094.x
JI Pitt (1979) The Genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces Academic Press London
AF Schindler AN Abadie RE Simpson (1980) ArticleTitleEnhanced aflatoxin production by Aspergillus flavus and Aspergillus parasiticus after gamma irradiation of the spore inoculum Journal of Food Protection 43 7–9 Occurrence Handle1:CAS:528:DyaL3cXht1Ojtbk%3D
A Torres ML Ramirez MM Reynoso M Rodriguez S Chulze (1997) ArticleTitleNatural co-occurrence of Fusarium species (section Liseola) and Aspergillus flavus group species, fumonisin and aflatoxin in Argentinian corn Cereal Research Communications 25 389–391
MW Trucksess ME Stack S Nesheim RH Albert TR Romer (1994) ArticleTitleMultifunctional column coupled with liquid chromatography for determination of aflatoxins B1, B2, G1, G2 in corn, almonds, Brazil nuts, peanuts and pistachio nuts: collaborative study Journal of Association Official Analytical Chemistry 6 1512–1521
DT Wicklow CW Hesseltine OL Shotwell GL Adams (1980) ArticleTitleInterference competition and aflatoxin levels in corn Phytopathology 70 761–764 Occurrence Handle1:CAS:528:DyaL3cXlsVKms7k%3D Occurrence Handle10.1094/Phyto-70-761
M Wilson SE Lindow (1994a) ArticleTitleCoexistence among epiphytic bacterial populations mediated through nutritional resource partitioning Applied and Environmental Microbiology 60 4468–4477 Occurrence Handle1:CAS:528:DyaK2MXitlynsro%3D
M Wilson SE Lindow (1994b) ArticleTitleEcological similarity and coexistence of epiphytic ice-nucleating (Ice+) Pseudomonas syringae strains and a non-ice-nucleating (Ice-) biological control agent Applied and Environmental Microbiology 60 3128–3137 Occurrence Handle1:CAS:528:DyaK2cXlslGjtLY%3D
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nesci, A.V., Bluma, R.V. & Etcheverry, M.G. In vitro Selection of Maize Rhizobacteria to Study Potential Biological Control of Aspergillus Section Flavi and Aflatoxin Production. Eur J Plant Pathol 113, 159–171 (2005). https://doi.org/10.1007/s10658-005-5548-3
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10658-005-5548-3