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Nicotine degradation enhancement by Pseudomonas stutzeri ZCJ during aging process of tobacco leaves

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Abstract

Nicotine is a key harmful component of tobacco and cigarettes, and the development of low-nicotine cigarettes is of increasing importance in the market. The objectives of this study are to isolate native nicotine-degrading strains and evaluate their feasibility for nicotine reduction during the aging (or fermentation) of tobacco leaves. A novel nicotine-degrading strain was isolated and identified as Pseudomonas stutzeri ZCJ based on its 16S rDNA sequence and morphological-biochemical characteristics. In submerged cultures, P. stutzeri ZCJ could tolerate 4.5 g/L nicotine and completely degrade 1.5 g/L nicotine within 24 h at 37°C and pH 7.4. The addition of glucose (1 g/L) could improve nicotine degradation by P. stutzeri ZCJ in submerged cultures. After submerged culturing, the cell suspension of P. stutzeri ZCJ could be utilized to improve nicotine reduction in tobacco leaves during solid-state fermentation. The nicotine content of tobacco leaves decreased by as much as 32.24% after 7 days of solid-state fermentation by P. stutzeri ZCJ, suggesting the industrial application potential of the native strain to enhance nicotine degradation during the aging of tobacco leaves.

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Abbreviations

HPLC:

High performance liquid chromatography

ESEM:

Environmental scanning electron microscope

rRNA:

Ribosomal ribonucleic acid

DNA:

Deoxyribonucleic acid

PCR:

Polymerase chain reaction

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Acknowledgments

We would like to acknowledge the Science and Technology Department of Zhejiang Province of PR China for their support under Grant No. 2007C23035.

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Authors and Affiliations

  1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Lei Zhao, Chenjing Zhu, Chang Wang, Xuanzhen Li & Weihong Zhong

  2. Technology Center, China Tobacco Zhejiang Industrial Co. Ltd., Hangzhou, 310009, People’s Republic of China

    Yang Gao & Ming Shu

  3. The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, People’s Republic of China

    Yuping Shi

Authors
  1. Lei Zhao
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  2. Chenjing Zhu
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  3. Yang Gao
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  4. Chang Wang
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  5. Xuanzhen Li
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  6. Ming Shu
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  7. Yuping Shi
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  8. Weihong Zhong
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Correspondence to Ming Shu, Yuping Shi or Weihong Zhong.

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Zhao, L., Zhu, C., Gao, Y. et al. Nicotine degradation enhancement by Pseudomonas stutzeri ZCJ during aging process of tobacco leaves. World J Microbiol Biotechnol 28, 2077–2086 (2012). https://doi.org/10.1007/s11274-012-1010-9

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  • DOI: https://doi.org/10.1007/s11274-012-1010-9

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