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Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sphingomonas sp. TY and their responses in the presence of neonicotinoid insecticides

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Abstract

Two novel nicotine-degrading bacterial strains were isolated from tobacco waste and identified as Acinetobacter sp. TW and Sphingomonas sp. TY based on morphology, physiological and biochemical tests, Biolog analysis and 16S rDNA sequencing. The 16S rDNA sequences have been deposited in GenBank under the accession numbers FJ753401 for TW and FJ754274 for TY. The best culture conditions for nicotine degradation were 25–37°C and pH 7.0–8.0 for strain TW and 25–30°C and pH 6.0–7.0 for strain TY. Under the best conditions, the cell growth and nicotine-degradation kinetics of the two isolates were assessed, and 1.0 g/l nicotine was completely degraded within 12 and 18 h for TW and TY, respectively. Moreover, the presence of four widely-used commercial neonicotinoid insecticides in the medium had no effects on nicotine degradation by TW; among the four tested neonicotinoids, only thiamethoxam significantly delayed nicotine degradation by TY. TW and TY were also able to degrade selected neonicotinoids. This is the first report of nicotine degradation by Acinetobacter sp. and Sphingomonas sp. This study showed that these two newly isolated bacteria may be suitable for the disposal of tobacco waste and the reduction of nicotine in tobacco leaves.

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Acknowledgments

This work was financially supported by the National Key Science and Technology Project: Water Pollution Control and Treatment (No.2008ZX07101-006), 863 High Technology Program (No. 2007AA10Z409), Innovative Research Team in Higher Educational Institutions of Zhejiang Province (T200912) and Open Fund of Key Lab of Environmental Pollution Control Technology in Zhejiang Province.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China

    Meizhen Wang

  2. College of Life Sciences, Zhejiang University, 310058, Hangzhou, China

    Guiqin Yang, Xin Wang, Yanlai Yao, Hang Min & Zhenmei Lu

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  1. Meizhen Wang
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  2. Guiqin Yang
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  3. Xin Wang
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Correspondence to Hang Min or Zhenmei Lu.

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Wang, M., Yang, G., Wang, X. et al. Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sphingomonas sp. TY and their responses in the presence of neonicotinoid insecticides. World J Microbiol Biotechnol 27, 1633–1640 (2011). https://doi.org/10.1007/s11274-010-0617-y

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