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Preparative separation of helvolic acid from the endophytic fungus Pichia guilliermondii Ppf9 by high-speed counter-current chromatography

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Abstract

High-speed counter-current chromatography (HSCCC) was applied for preparative separation of helvolic acid from the crude extract of the endophytic fungus Pichia guilliermondii Ppf9, associated with the medicinal plant Paris polyphylla var. yunnanensis for the first time. The two-phase solvent system consisted of n-hexane–ethyl acetate–methanol–water (4.5:4.5:5.0:5.0, v/v) appending with phosphoric acid (0.2%, v/v) was employed. The revolution speed of the separation column, flow rate of the mobile phase and separation temperature of the apparatus were 800 rpm, 3 ml min−1 and 25°C, respectively. About 6.8 mg of helvolic acid was successfully obtained from 450 mg of the crude extract by HSCCC within 4 h separation procedure, and its purity reached to 93.2% according to the HPLC analysis. The product was further characterized by MS, 1H-NMR and 13C-NMR spectra.

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Acknowledgments

This work was co-financed by the grants from the Hi-Tech R&D Program of China (2011AA10A202), the National Basic Research Program of China (2010CB126105), and the National Natural Science Foundation of China (30871662 and 31071710).

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Authors and Affiliations

  1. Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China

    Jianglin Zhao, Yan Mou, Tijiang Shan, Yan Li, Shiqiong Lu & Ligang Zhou

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  1. Jianglin Zhao
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  2. Yan Mou
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  3. Tijiang Shan
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  4. Yan Li
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  5. Shiqiong Lu
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  6. Ligang Zhou
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Correspondence to Ligang Zhou.

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Zhao, J., Mou, Y., Shan, T. et al. Preparative separation of helvolic acid from the endophytic fungus Pichia guilliermondii Ppf9 by high-speed counter-current chromatography. World J Microbiol Biotechnol 28, 835–840 (2012). https://doi.org/10.1007/s11274-011-0878-0

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