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Endophytic fungi from rhizomes of Paris polyphylla var. yunnanensis

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Abstract

About 63 fungal endophytic isolates were separated from rhizomes of Paris polyphylla var. yunnanensis, which is a traditional medicinal plant mainly distributed in China. The isolates were characterized and grouped based on the culture characteristics and the morphology of colony growth and conidia. Eleven representative ones were selected for further taxonomical identification. Five genera namely Fusarium, Gliocladiopsis, Gliomastix, Aspergillus and Cylindrocarpon were identified on the basis of their morphological characterizations. Of them, the most frequent genus was Fusarium (i.e. Ppf1, Ppf3 and Ppf14). Their ITS-rDNA sequences were compared with those available in the GeneBank databases to obtain the closest related species by BLAST analysis as well as to analyze their phylogenetic affiliation. The isolates were identified as Gliocladiopsis irregularis (Ppf2), Plectosphaerella cucumerina (Ppf4), Padospora sp. (Ppf6), Gliomastix murorum var. murorum (Ppf7), Aspergillus fumigatus (Ppf9), Pichia guilliermondii (Ppf10), Neonectria radicicola (anamorph: Cylindrocarpon) (Ppf12) and one uncultured mycorrhizal ascomycete (Ppf13) separately based on their morphological and molecular features. The molecular characters of the endophytic fungi were basically coincident with their morphology. The broad diversity and taxonomic spectrum were exhibited by the endophytic fungi from P. polyphylla var. yunnanensis.

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Acknowledgements

This work was co-financed by the grants from the Hi-Tech Research and Development Program of China (No. 2006AA10Z423) and National Natural Science Foundation of China (No. 30470038). The authors are grateful to Prof. Shilin Wang of Kunming Institute of Botany in Chinese Academy of Sciences for plant material collection and identification.

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Authors and Affiliations

  1. Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100094, China

    Jing Li, Jianglin Zhao, Lijian Xu & Ligang Zhou

  2. College of Resources and Environment, China Agricultural University, Beijing, 100094, China

    Xiaolin Li & Jingguo Wang

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  1. Jing Li
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  2. Jianglin Zhao
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  3. Lijian Xu
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  4. Ligang Zhou
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  5. Xiaolin Li
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  6. Jingguo Wang
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Correspondence to Ligang Zhou.

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Li, J., Zhao, J., Xu, L. et al. Endophytic fungi from rhizomes of Paris polyphylla var. yunnanensis . World J Microbiol Biotechnol 24, 733–737 (2008). https://doi.org/10.1007/s11274-007-9531-3

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  • DOI: https://doi.org/10.1007/s11274-007-9531-3

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