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Simultaneous separation and purification of calycosin and formononetin from crude extract of Astragalus membranaceus Bge. var. mongholicus (Bge.) using high-speed counter-current chromatography

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Abstract

A simple and effective high-speed counter-current chromatography (HSCCC) method for simultaneous separation and purification of calycosin and formononetin from the ethanolic crude extract of Astragalus membranaceus is described. HSCCC separation was performed using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (2.5: 2.5: 2: 3, v/v). A total of 7.5 mg calycosin and 11.2 mg formononetin was obtained from 500 mg crude extract of Astragalus membranaceus with purities of 98.5 and 98.3%, respectively, determined using HPLC. Chemical structures were confirmed by MS, 1HNMR.

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Authors and Affiliations

  1. College of Science, Hunan Agricultural University Changsha, Changsha, 410128, P.R. China

    Xia Li, Xinrong Dong & Ming Shuai

  2. College of Chemistry and Chemical Engineering, Research Center of Modernization of Chinese Medicines, Central South University, Changsha, 410083, P.R. China

    Xinrong Dong & Yizeng Liang

Authors
  1. Xia Li
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  2. Xinrong Dong
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  3. Ming Shuai
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  4. Yizeng Liang
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Correspondence to Xia Li.

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Li, X., Dong, X., Shuai, M. et al. Simultaneous separation and purification of calycosin and formononetin from crude extract of Astragalus membranaceus Bge. var. mongholicus (Bge.) using high-speed counter-current chromatography. J Anal Chem 70, 92–97 (2015). https://doi.org/10.1134/S1061934815010207

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  • DOI: https://doi.org/10.1134/S1061934815010207

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