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Organosolvent pretreatment and enzymatic hydrolysis of rice straw for the production of bioethanol

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Abstract

The present study investigates the operational conditions for organosolvent pretreatment and hydrolysis of rice straw. Among the different organic acids and organic solvents tested, acetone was found to be most effective based on the fermentable sugar yield. Optimization of process parameters for acetone pretreatment were carried out. The structural changes before and after pretreatment were investigated by scanning electron microscopy, X-ray diffraction and Fourier transform infrared (FTIR) analysis. The X-ray diffraction profile showed that the degree of crystallinity was higher for acetone pretreated biomass than that of the native. FTIR spectrum also exhibited significant difference between the native and pretreated samples. Under optimum pretreatment conditions 0.458 g of reducing sugar was produced per gram of pretreated biomass with a fermentation efficiency of 39%. Optimization of process parameters for hydrolysis such as biomass loading, enzyme loading, surfactant concentration and incubation time was done using Box–Benhken design. The results indicate that acetone pretreated rice straw can be used as a good feed stock for bioethanol production.

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References

  • Alvira P, Ballesteros TM, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis. A review. Bioresour Technol 101:4851–4861

    Article  CAS  Google Scholar 

  • Araque E, Parra C, Freer J, Contreras D, Rodriguez J, Mendonca R, Baeza J (2008) Evaluation of organosolv pretreatment for the conversion of Pinus radiata D. Don to ethanol. Enzyme Microb Technol 43:214–219

    Article  CAS  Google Scholar 

  • Aswathy US, Sukumaran RK, Devi GL, Rajasree KP, Singhania RR, Pandey A (2010) Bioethanol from water hyacinth biomass: an evaluation of enzymatic saccharification strategy. Bioresour Technol 101:925–930

    Article  CAS  Google Scholar 

  • Bak SJ, Ja KK, Young HH, Byung CL, Choi IG, Kim KH (2009) Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation. Bioresour Technol 100:1285–1290

    Article  CAS  Google Scholar 

  • Binod P, Sindhu R, Singhania RR, Surender JS, Devi L, Nagalakshmi S, Kurien N, Sukumaran RK, Pandey A (2010) Bioethanol production from rice straw: an overview. Bioresour Technol 101:4767–4774

    Article  CAS  Google Scholar 

  • Binod P, Janu KU, Sindhu R, Pandey A. In: Pandey A, Larroche C, Ricke SC (eds) Biofuels: alternative feedstocks and conversion processes. Elsevier, USA (in press)

  • Box GEP, Behnken DW (1960) Some new three level designs for the study of quantitative variables. Technometrics 2:455–475

    Article  Google Scholar 

  • Cao Y, Tan H (2004) Structural characterization of cellulose with enzymatic treatment. J Mol Struct 705:189–193

    Article  CAS  Google Scholar 

  • Chen M, Xia LM, Xue PJ (2007) Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate. Int Biodeter Biodegr 59:85–89

    Article  CAS  Google Scholar 

  • Duff SJB, Murray WD (1996) Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. Bioresour Technol 55:1–33

    Article  CAS  Google Scholar 

  • Eggerman T, Elander RT (2005) Process and economic analysis of pretreatment technologies. Bioresour Technol 96:2019–2025

    Article  Google Scholar 

  • Gnansounou E (2009) In: Pandey A (ed) Hand book of plant-based biofuels—fuel ethanol current status and outlook. CRC Press, pp 57–71

  • Hinterstoisser B, Akerholm M, Salmen L (2001) Effect of fiber orientation in dynamic FTIR study on native cellulose. Carbohydr Res 334:27–37

    Article  CAS  Google Scholar 

  • Huijgen WJJ, Vanderlaan RR, Reith JH (2008) Modified organosolv as a fractionation process of lignocellulosic biomass for coproduction of fuels and chemicals. 16th European Biomass Conference and Exhibition, Valencia, Spain, June 2–6

  • Itoh H, Wada M, Honda Y, Kuwahara M, Watanabe T (2003) Bioorganosolv pretreatments for simultaneous saccharification and fermentation of beech wood by ethanolysis and white rot fungi. J Biotechnol 103:273–280

    Article  CAS  Google Scholar 

  • Jahan MS, Lee ZZ, Jin Y (2006) Organic acid pulping of rice straw I: cooking. Turk J Agric For 30:231–239

    CAS  Google Scholar 

  • Jamshid MR, Alireza T, Pejman RC (2005) Pulping of rice straw by high boiling point solvents in atmospheric pressure. Iran Polym J 14:223–227

    Google Scholar 

  • Jin S, Chen H (2006) Superfine grinding of steam—exploded rice straw and its enzymatic hydrolysis. Biochem Eng J 30:225–230

    Article  CAS  Google Scholar 

  • Kim S, Holtzapple MT (2006) Effect of structural features on enzyme digestibility of corn stover. Bioresour Technol 97:583–591

    Article  CAS  Google Scholar 

  • Kim TH, Lee YY (2007) Pretreatment of corn stover by soaking in aqueous ammonia at moderate temperatures. Appl Biochem Biotechnol 36:81–92

    Article  Google Scholar 

  • Ko JK, Bak JS, Jung MW, Lee HJ, Choi IG, Kim TH, Kim KH (2009) Ethanol production from rice straw using optimized aqueous ammonia soaking pretreatment and simultaneous saccharification and fermentation process. Bioresour Technol 100:4374–4380

    Article  CAS  Google Scholar 

  • Kootstra MJ, Beeftink HH, Scott EL, Sanders JPM (2009) Comparison of dilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheat straw. Biochem Eng J 46:126–131

    Article  CAS  Google Scholar 

  • Kumar R, Wyman CE (2009) Does change in accessibility with conversion depend on both the substrate and pretreatment technology? Bioresour Technol 100:4193–4202

    Article  CAS  Google Scholar 

  • Kun N, Peng C, Xu Z, Wen-Song T (2009) Enhanced enzymatic hydrolysis of rice straw pretreated by alkali assisted with photocatalysis technology. J Chem Technol Biotechnol 84:1240–1245

    Article  Google Scholar 

  • Li H, Kim HJ, Jiang M, Kang JW, Chang HO (2009) Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid–acetone for bioethanol production. Bioresour Technol 100:3245–3251

    Article  CAS  Google Scholar 

  • Liu CF, Xu F, Sun JX, Ren JL, Curling S, Sun RC, Fowler P, Baird MS (2006) Physicochemical characterisation of cellulose from perennial ryegrass leaves (Lolium perenne). Carbohydr Res 341:2677–2687

    Article  CAS  Google Scholar 

  • Lu XB, Zhang YM, Yang J, Liang Y (2007) Enzymatic hydrolysis of corn stover after pretreatment with dilute sulphuric acid. Chem Eng Technol 30:938–944

    Article  CAS  Google Scholar 

  • Mansfield SD, Mooney C, Saddler JN (1999) Substrate and enzyme characteristics that limit cellulose hydrolysis. Biotechnol Prog 15:804–816

    Article  CAS  Google Scholar 

  • Miller GM (1959) Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Moxley GM, Zhu Z, Zhang HP (2008) Efficient sugar release by the cellulose solvent based lignocellulose fractionation technology and enzymatic cellulose hydrolysis. J Agri Food Chem 56:7885–7890

    Article  CAS  Google Scholar 

  • Mussato SI, Dragone G, Fernandes M, Milagres AMF, Roberto IC (2008) The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain. Cellulose 15:711–721

    Article  Google Scholar 

  • Nguyen TA, Kim KR, Han SJ, Cho HY, Kim JW, Park SM, Park JC, Sim SJ (2010) Pretreatment of rice straw with ammonia and ionic liquid for lignocellulose conversion to fermentable sugars. Bioresour Technol 101:7432–7438

    Article  CAS  Google Scholar 

  • Noureddini H, Byun J (2010) Dilute acid pretreatment of distiller’s grains and corn fiber. Bioresour Technol 101:1060–1067

    Article  CAS  Google Scholar 

  • Oh SY, Yoo DI, Shin Y, Kim HC, Kim HY, Chung YS, Park WH, Youk JH (2005) Crystalline structure analysis of cellulose treated with sodium hydroxide and carbon dioxide by means of X-ray diffraction and FTIR spectroscopy. Carbohydr Res 340:2376–2391

    Article  CAS  Google Scholar 

  • Pan XJ, Sano Y (1999) Atmospheric acetic acid pulping of rice straw IV: physico- chemical characterization of acetic acid lignins from rice straw and woods. Holzforschung 53:590–596

    Article  CAS  Google Scholar 

  • Pan XJ, Arato C, Gilkes N, Gregg DJ, Mabee W, Pye EK, Xiao Z, Zhang X, Saddler JN (2005) Biorefining of softwoods using ethanol organosolv pulping—preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products. Biotechnol Bioeng 90:473–481

    Article  CAS  Google Scholar 

  • Pan X, Gilkes N, Kadla J, Pye K, Saka S, Gregg D, Ehara K, Xie D, Lam D, Saddler J (2006) Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation process: optimization of process yields. Biotechnol Bioeng 94:851–861

    Article  CAS  Google Scholar 

  • Paszner L, Quinde AA, Meshgini M (1985) ACOS—accelerated hydrolysis of wood by acid catalysed organosolv means. International Symposium on Wood and Pulping Chemistry, Vancouver, Canada, pp 235–240

  • Satyanagalakshmi K, Sindhu R, Binod P, Janu KU, Sukumaran RK, Pandey A (2011) Bioethanol production from acid pretreated water hyacinth by separate hydrolysis and fermentation. J Sci Ind Res 70:156–161

    CAS  Google Scholar 

  • Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, Osborne JA (2007) Comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresour Technol 98:3000–3011

    Article  CAS  Google Scholar 

  • Sindhu R, Binod P, Nagalakshmi S, Janu KU, Sajna KV, Kurien N, Sukumaran RK, Pandey A (2010) Formic acid as a potential pretreatment agent for conversion of sugarcane bagasse to ethanol. Appl Biochem Biotechnol 162:2313–2323

    Article  CAS  Google Scholar 

  • Sumphanwanich J, Leepipatpiboon N, Srinorakutara T, Akaracharanya A (2008) Evaluation of dilute-acid pretreated bagasse, corn cob and rice straw for ethanol fermentation by Saccharomyces cerevisiae. Ann Microbiol 58:219–225

    Article  CAS  Google Scholar 

  • Sun F, Chen HZ (2008) Organosolv pretreatment by crude glycerol from oleochemicals industry for enzymatic hydrolysis of wheat straw. Bioresour Technol 99:5474–5479

    Article  CAS  Google Scholar 

  • Sun Y, Cheng J (2002) Hydrolysis of lignocellulosics materials for ethanol production: a review. Bioresour Technol 83:1–11

    Article  CAS  Google Scholar 

  • Sun Y, Lin L, Pang C, Deng H, Peng H, Li J, He B, Liu S (2007) Hydrolysis of cotton fiber cellulose in formic acid. Energy Biofuels 21:2386–2389

    Article  CAS  Google Scholar 

  • Taniguchi M, Suzuki H, Watanabe D, Sakai K, Hoshino K, Tanaka T (2005) Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw. J Biosci Bioeng 100:637–643

    Article  CAS  Google Scholar 

  • Tengborg C, Galbe M, Zacchi G (2001) Influence of enzyme loading and physical parameters on the enzymatic hydrolysis of steam—pretreated soft wood. Biotechnol Progr 17:110–117

    Article  CAS  Google Scholar 

  • Wei CJ, Cheng CY (1985) Effect of hydrogen peroxide pretreatment on the structural features and the enzymatic hydrolysis of rice straw. Biotechnol Bioeng 27:1418–1426

    Article  CAS  Google Scholar 

  • Weil JR, Dien B, Bothast R, Hendrickson R, Mosier NS, Ladisch MR (2002) Removal of fermentation inhibitors formed during pretreatment of biomass by polymeric adsorbants. Ind Eng Chem Res 41:6132–6138

    Article  CAS  Google Scholar 

  • Xu Z, Wang Q, Jiang Z, Yang X, Ji Y (2007) Enzymatic hydrolysis of pretreated soybean straw. Biomass Bioenergy 31:162–167

    Article  CAS  Google Scholar 

  • Zhang QZ, Cai WM (2008) Enzymatic hydrolysis of alkali pretreated rice straw by Trichoderma reesei ZM4-F3. Biomass Bioenergy 32:1130–1135

    Article  CAS  Google Scholar 

  • Zhang YHP, Ding SY, Mielenz JR, Elander R, Laser M, Himmel M, McMillan JD, Lynd LR (2007) Fractionating recalcitrant lignocellulose at modest reaction conditions. Biotechnol Bioeng 97:214–223

    Article  CAS  Google Scholar 

  • Zhao X, Cheng K, Liu D (2009) Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis. Appl Microbiol Biotechnol 82:815–827

    Article  CAS  Google Scholar 

  • Zhu SM, Wu Y, Yu Z, Wang C, Yu F, Jin S, Ding Y, Chi R, Liao J, Zhang Y (2005) Comparison of three microwave/chemical pretreatment processes for enzymatic hydrolysis of rice straw. Biosyst Eng 93:279–283

    Article  Google Scholar 

  • Zhu L, Odwyer JP, Chang VS, Granda CB, Holtzapple MT (2010) Multiple linear regression models for predicting biomass digestibility from structural features. Bioresour Technol 101:4971–4979

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to Technology Information, Forecasting and Assessment Council (TIFAC), Department of Science and Technology, Government of India and Council of Scientific and Industrial Research (CSIR), New Delhi for financial support to Centre for Biofuels.

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Authors and Affiliations

  1. Centre for Biofuels, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum, 695 019, India

    Raveendran Sindhu, Parameswaran Binod, Kanakambaran Usha Janu, Rajeev K. Sukumaran & Ashok Pandey

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  1. Raveendran Sindhu
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  2. Parameswaran Binod
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  3. Kanakambaran Usha Janu
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  4. Rajeev K. Sukumaran
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  5. Ashok Pandey
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Correspondence to Ashok Pandey.

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Sindhu, R., Binod, P., Janu, K.U. et al. Organosolvent pretreatment and enzymatic hydrolysis of rice straw for the production of bioethanol. World J Microbiol Biotechnol 28, 473–483 (2012). https://doi.org/10.1007/s11274-011-0838-8

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  • DOI: https://doi.org/10.1007/s11274-011-0838-8

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