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Production of phytohormones by root-associated saprophytic actinomycetes isolated from the actinorhizal plant Ochetophila trinervis

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Abstract

The aim of the present study was to evaluate phytohormone production by symbiotic and saprophytic actinomycetes isolated from the actinorhizal plant Ochetophila trinervis which had previously proved to stimulate nodulation by Frankia. Three saprophytic strains out of 122, isolated from the rhizosphere of this plant with multiple enzymatic activities were selected for plant growth experiments in pots: Streptomyces sp. (BCRU-MM40), Actinoplanes sp. (BCRU-ME3) and Micromonospora sp. (BCRU-MM18). For experiments, the symbiotic N2-fixing strain Frankia (BCU110501), isolated from nodules of the same actinorhizal plant was used. Phytohormone production was evaluated in supernatant of non-inoculated and inoculated culture media in exponential growth phase. Indole 3-acetic acid (IAA) and gibberellic acid (GA3) were analyzed by gas chromatography-mass spectrometry (GC–MS), while zeatine (Z) production was determined by gas chromatography-flame ionization detector and high performance liquid chromatography (HPLC fluorescent and UV). The levels of the three phytohormones produced by the saprophytic rhizoactinomycetes were higher than that produced by the symbiotic Frankia strain. Zeatine biosynthesis was higher (μg ml−1) than IAA and GA3 (ng ml−1), and Micromonospora strain produced the highest levels of these phytohormones. Although O. trinervis has been shown to be intercellularly infected by Frankia without mediation of root hair deformation, when plants were co-inoculated with actinomycetes’ culture, some root hair deformation was observed. This is the first report on identification of IAA, GA3 and Z in saprophytic actinomycetes and their potential role in plant–microbe interaction.

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Acknowledgments

Financial support for this study was provided through grants by Universidad Nacional del Comahue 04/B118 and Agencia Nacional de Promoción Científica y Tecnológica PICT 10006 and PICT 20568, Universidad Nacional de Quilmes, all from Argentina. F. Cassán, V. Luna and L. G. Wall are members of CONICET.

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Authors and Affiliations

  1. Dpto. Botánica, CRUB-UNComahue, Quintral 1250, 8400 S.C., Bariloche, Argentina

    Mariana Solans & Gernot Vobis

  2. Lab. Fisiología Vegetal, Dpto. Cs. Naturales, UNRC, 5800, Río Cuarto, Argentina

    Fabricio Cassán & Virginia Luna

  3. Dpto. Ciencia y Tecnología, UNQ, R. Sáenz Peña 352, B1876BXD, Bernal, Argentina

    Luis Gabriel Wall

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  1. Mariana Solans
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  2. Gernot Vobis
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  3. Fabricio Cassán
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  4. Virginia Luna
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  5. Luis Gabriel Wall
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Correspondence to Mariana Solans.

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Solans, M., Vobis, G., Cassán, F. et al. Production of phytohormones by root-associated saprophytic actinomycetes isolated from the actinorhizal plant Ochetophila trinervis . World J Microbiol Biotechnol 27, 2195–2202 (2011). https://doi.org/10.1007/s11274-011-0685-7

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