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Bioactivity and genome analysis of Bacillus amyloliquefaciens GL18 isolated from the rhizosphere of Kobresia myosuroides in an alpine meadow

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Abstract

The unique eco-environment of the Qinghai–Tibet Plateau breeds abundant microbial resources. In this research, Bacillus amyloliquefaciens GL18, isolated from the rhizosphere of Kobresia myosuroides from an alpine meadow, and the antagonistic activity, bacteriostatic hydrolase activity, and low temperature, salt, and drought resistance of it were determined and analysed. The seedlings of Avena sativa were root-irrigated using bacteria suspensions (cell concentration 1 × 107 cfu/mL) of GL18, and the growth-promoting effect of GL18 on it was determined under cold, salt and drought stress, respectively. The whole genome of GL18 was sequenced, and its functional genes were analysed. GL18 presented significant antagonistic activity to Fusarium graminearum, Fusarium acuminatum, Fusarium oxysporum and Aspergillus niger (inhibition zone diameter > 17 mm). Transparent zones formed on four hydrolase detection media, indicating that GL18 secreted cellulase, protease, pectinase and β-1,3-glucanase. GL18 tolerated conditions of 10 °C, 11% NaCl and 15% PEG-6000, presenting cold, salt and drought resistance. GL18 improved the cold, salt and drought tolerance of A. sativa and it showed significant growth effects under different stress. The total length of the GL18 genome was 3,915,550 bp, and the number of coding DNA sequence was 3726. Compared with the clusters of orthologous groups of proteins, gene ontology and kyoto encyclopedia of genes and genomes databases, 3088, 2869 and 2357 functional genes were annotated, respectively. GL18 contained gene clusters related to antibacterial substances, functional genes related to the synthesis of plant growth-promoting substances, and encoding genes related to stress resistance. This study identified an excellent Bacillus strain and provided a theoretical basis for improving stress resistance and promoting the growth of herbages under abiotic stress.

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Data availability

The datasets generated during and analyzed during the current study are available in the NCBI repository, https://www.ncbi.nlm.nih.gov/nuccore/CP096033.1/.

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Acknowledgements

Authors are grateful to the authorities of respective departments for support in doing this research. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

This work was funded by National Natural Science Foundation of China (32160030), International Sci-technology Cooperation project of Qinghai Provincial Department of Science and Technology (2018-HZ-813), State Key Laboratory of Ecology and Plateau Agriculture and Animal Husbandry, Qinghai University (2019-ZZ-12), and Applied Basic Research Project of Qinghai Provincial Department of Science and Technology (2017-ZJ-788).

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Authors and Affiliations

  1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China

    L. Chen, Y. L. Xie, X. H. Wu, L. L. Wu & F. Yang

  2. College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China

    L. Chen, Y. L. Xie, X. H. Wu, L. L. Wu, J. Yang, Y. Gao, Y. Mi & F. Yang

  3. Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai University, Xining, 810016, China

    Y. L. Xie

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  1. L. Chen
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Contributions

Author YX designed the study and administrated the project. LC performed the experiments, analysed data, and wrote the draft version of the manuscript. LC and XW collected samples for whole-genome sequencing and analysed the genome data. LW, JY and YG detected the antagonistic activity, bacteriostatic hydrolase activity, and low temperature, salt, and drought resistance. YM and FY detected the growth-promoting effect of A. sativa. under cold, salt and drought stress. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Y. L. Xie.

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Chen, L., Xie, Y.L., Wu, X.H. et al. Bioactivity and genome analysis of Bacillus amyloliquefaciens GL18 isolated from the rhizosphere of Kobresia myosuroides in an alpine meadow. Antonie van Leeuwenhoek 117, 16 (2024). https://doi.org/10.1007/s10482-023-01917-x

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