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Arbuscular mycorrhizal fungal community structure and diversity in response to long-term fertilization: a field case from China

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Abstract

The influences of different fertilizer treatments on spore community structure and diversity of arbuscular mycorrhizal (AM) fungi (AMF) were investigated in a long-term fertilization experiment with seven treatments: organic manure (OM), half organic manure N plus half fertilizer N (1/2 OMN), fertilizer NPK, fertilizer NP, fertilizer NK, fertilizer PK, and the control (without fertilization). Fertilization generally increased the nutrient contained in the fertilizer and treatments with NPK and 1/2 OMN produced the highest crop yields. Thirty-five species of AMF within 6 genera, including 8 previously undescribed species, were recovered. Similarly in all seven treatments, the most abundant genus was Glomus, and followed by Acaulospora. All the fertilization treatments changed AM species composition, and NK treatment had the slightest influence. Fertilization with fertilizers NP, PK and NPK markedly increased AM fungal spore density, while 1/2 OMN, OM and NK treatments showed no significant influences. All the fertilizer treatments, especially OM, significantly decreased species richness and species diversity (Shannon-Weiner index). There were no significant correlations between AM fungal parameters (spore density, species richness and species diversity) and soil properties. The findings indicate that long-term fertilization all can change AM fungal community structure and decrease species diversity, while balanced fertilization with NPK or 1/2 OMN is the most suitable fertilization regime if taking both crop yields and AM species diversity into account.

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Acknowledgments

We thank Jia Bao Zhang, Lin Yun Zhou, Qi Ao Jiang and Jian Liu, of the Fengqiu Agro-Ecological Experimental Station, Institute of Soil Science, Chinese Academy of Sciences, for their excellent field management and kind support on soil sample collection. This work is supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. ISSASIP0703, Kzcx2-yw-408, Kscx1-yw-09-05) and National Basic Research Program of China (Project no. 2005CB121108).

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Authors and Affiliations

  1. State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hongkong Baptist University & Institute of Soil Science, Chinese Academy of Sciences, East Beijing Road 71, Nanjing, 210008, People’s Republic of China

    Fa Yuan Wang, Jun Li Hu, Xian Gui Lin, Sheng Wu Qin & Jun Hua Wang

  2. Agricultural College, Henan University of Science and Technology, Luoyang, Henan Province, 471003, People’s Republic of China

    Fa Yuan Wang

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  1. Fa Yuan Wang
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  2. Jun Li Hu
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  3. Xian Gui Lin
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  5. Jun Hua Wang
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Correspondence to Xian Gui Lin.

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Wang, F.Y., Hu, J.L., Lin, X.G. et al. Arbuscular mycorrhizal fungal community structure and diversity in response to long-term fertilization: a field case from China. World J Microbiol Biotechnol 27, 67–74 (2011). https://doi.org/10.1007/s11274-010-0427-2

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  • DOI: https://doi.org/10.1007/s11274-010-0427-2

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