Abstract
A thermostable cellulase-producing fungus, HML 0278, was identified as Fusarium chlamydosporum by morphological characteristics and ITS rDNA sequence analysis. HML 0278 produced extracellular cellulases in solid-state fermentation using sugar cane bassage as the carbon source. Native-PAGE analysis demonstrated that this fungus strain was capable of producing the three major components of cellulases and xylanase, with a yield of 281.8 IU/g for CMCase, 182.4 IU/g for cellobiohydrolase, 135.2 IU/g for β-glucosidase, 95.2 IU/g for filter paper activity, and 4,720 IU/g for xylanase. More importantly, the CMCase and β-glucosidase produced by HML 0278 showed stable enzymatic activities within pH 4–9 and pH 4–10, and at temperatures below 70 and 60°C, respectively.
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This work was supported in part by the National Natural Science Foundation of China (20066001), Guangxi Natural Science Foundation (2010GXNSFA013103), and by the Open Fund of Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization (SB0607).
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Qin, Y., He, H., Li, N. et al. Isolation and characterization of a thermostable cellulase-producing Fusarium chlamydosporum . World J Microbiol Biotechnol 26, 1991–1997 (2010). https://doi.org/10.1007/s11274-010-0383-x
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DOI: https://doi.org/10.1007/s11274-010-0383-x