Abstract
A Streptomyces sp. was isolated that produced novel thermoalkalotolerant cellulase activity after growth on crystalline cellulose at 50°C. Three major components of the cellulases (CMCase, Avicelase and cellobiase) were produced with maximal activities (11.8, 7.8 and 3.9 IU/ml) and maximum specific activities 357, 276 and 118 IU/mg protein, respectively, after 120 h growth. Maximum CMCase activity was between 50 and 60°C measured over 3 h. The enzyme also retained 88% of its maximum activity at 70°C and pH 5, and 80% of the activity at pH 10 and 50°C when assayed after 1 h. After incubation at 40°C for 1 h with commercial detergent (Tide) at pH 11, 95% activity was retained. The enzyme mixture produced glucose from crystalline cellulose.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chand P, Aruna A, Maqsood A, Rao L (2005) Novel mutation method for increased cellulase production. J Appl Microbiol 98:318–323
George S, Ahmad A, Rao M (2001) Studies on carboxymethyl cellulase produced by an alkalothermophilic actinomycete. Bioresource Technol 77:171–175
Ghose T (1987) Measurement of cellulase activities. Pure & Appl Chem 59:257–268
Jang H, Chen K (2003) Production and characterization of thermostable cellulases from Streptomyces transformant T3–1. World J Microbiol Biotechnol 19:263–268
Jang H, Chang K (2005) Thermostable cellulases from Streptomyces sp: scale-up production in 50 l fermenter. Biotechnol Lett 27:239–242
Lima A, Nascimento R, Bon E, Coelho R (2005) Streptomyces drozdowiczii cellulase production using agro-industrial by-products and its potential use in the detergent and textile industries. Enzyme Microbial Technol 37:272–277
Locci R (1984) Streptomycetes and related genera. In: Williams S (ed) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2451–2508
MacDonald D, Bakhshi N, Mathews J, Roychowdhury A, Bajpai P, Moo-Young M (1983) Alkali treatment of corn stover to improve sugar production by enzymatic hydrolysis. Biotechnol Bioeng 25:2067–2076
Nakai R, Horinouchi S, Uozumi T, Beppu T (1987) Purification and properties of cellulases from an alkalophilic Streptomyces strain. Agric Biol Chem 51:3061–3065
Semedo L, Gomes R, Bon E, Soares R, Linhares L, Coelho R (2000) Endocellulase and exocellulase activities of two Streptomyces strains isolated from a forest soil. Appl Biochem Biotechnol 84:267–276
Zhang Y, Himmel M, Mielenz J (2006) Outlook for cellulase improvement: Screening and selection strategies. Biotechnol Adv 24:452–481
Acknowledgments
Portions of this research were funded through the Natural Sciences and Engineering Research Council of Canada.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Alani, F., Anderson, W.A. & Moo-Young, M. New isolate of Streptomyces sp. with novel thermoalkalotolerant cellulases. Biotechnol Lett 30, 123–126 (2008). https://doi.org/10.1007/s10529-007-9500-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-007-9500-9