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Bioactive metabolites from Penicillium sp., an endophytic fungus residing in Hopea hainanensis

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Abstract

The metabolites of endophytic fungus Penicillium sp. from the leaf of Hopea hainanensis were reported for the first time. By bioassay-guided fractionation, the EtOAc extract of a solid-matrix steady culture of this fungus afforded six compounds, which were identified through a combination of spectral and chemical methods (IR, MS, 1H- and 13C-NMR) to be monomethylsulochrin (1), rhizoctonic acid (2), asperfumoid (3), physcion (4), 7,8-dimethyl-iso-alloxazine (5) and 3,5-dichloro-p-anisic acid (6). Compounds 2, 3 and 6 were obtained from Penicillium sp. for the first time. All of the six isolates were subjected to in vitro bioactive assays including antifungal action against three human pathogenic fungi Candida albicans, Trichophyton rubrum and Aspergillus niger and cytotoxic activity against the human nasopharyngeal epidermoid tumor KB cell line and human liver cancer HepG2 cell line. As a result, compounds 2–4 and 6 inhibited the growth of C. albicans with MICs of 40.0, 20.0, 50.0 and 15.0 μg/ml, respectively and the compound 6 showed growth inhibition against A. niger with MICs of 40.0 μg/ml. In addition, compounds 1–3 and 6 exhibited cytotoxic activity against KB cell line with IC50 value of 30.0, 20.0, 20.0, 5.0 μg/ml, respectively and against HepG2 cell line with IC50 value of 30.0, 25.0, 15.0, 10.0 μg/ml, respectively.

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Acknowledgements

The work was co-supported by NSFC (20432030) and by QDNSF (06-2-2-15-jch).

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Authors and Affiliations

  1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, 266109, P.R. China

    F. W. Wang, C. R. Wang & P. Li

  2. Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu, 210093, P.R. China

    F. W. Wang & D. H. Shi

  3. College of Aquaculture, Qingdao Agricultural University, Qingdao, Shandong, 266109, P.R. China

    Z. M. Hou

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  1. F. W. Wang
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  2. Z. M. Hou
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  3. C. R. Wang
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  4. P. Li
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  5. D. H. Shi
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Correspondence to F. W. Wang.

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Wang, F.W., Hou, Z.M., Wang, C.R. et al. Bioactive metabolites from Penicillium sp., an endophytic fungus residing in Hopea hainanensis . World J Microbiol Biotechnol 24, 2143–2147 (2008). https://doi.org/10.1007/s11274-008-9720-8

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  • DOI: https://doi.org/10.1007/s11274-008-9720-8

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