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Treatment of Paenibacillus illinoisensis suppresses the activities of antioxidative enzymes in pepper roots caused by Phytophthora capsici infection

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Summary

The activity of antioxidative enzymes after inoculation of pepper (Capsicum annuum L. Chungok) with a pathogen, Phytophthora capsici (P), the causal agent of Phytophtora blight and dual inoculation of pathogen and an antagonist, Paenibacillus illinoisensis KJA-424 (P+A), were measured and compared with that of non-inoculated (C) roots. Root mortality was significantly reduced by about 84% in P+A treatment compared with P treatment alone. When compared to the non-inoculated (C) roots, malondialdehyde (MDA) concentration gradually decreased by 52.4% in 7 days only in P-treated roots and hydrogen peroxide (H2O2) was not significantly affected by the treatment for 5 days but significantly decreased in the P+A-treated roots at day 7. P-treatment continuously induced peroxidase (POD) and superoxide dismutase (SOD), resulting in significant increases of 36.7% and 27.7% at day 7, respectively, compared to the control. In P+A-treated roots, the activities of POD and SOD also increased for 5 days but returned to the control level at day 7. Catalase activity fluctuated but again increased over the 7-day period following P+A inoculation. These results indicate that an antagonist P. illinoisensis KJA-424 alleviated root mortality and suppressed the elevated activities of POD and SOD in the root of pepper plant root caused by P.␣capsici infection.

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Acknowledgements

This work was supported by National Research Laboratory (NRL) program (to Dr R. D. Park) from the Ministry of Science & Technology (MOST), and by Korea Science and Engineering Foundation (KOSEF) through the Agricultural Plants Stress Research Center (APSRC) at Chonnam National University.

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Authors and Affiliations

  1. Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, and APSRC, College of Agriculture & Life Science, Chonnam National University, 500-757, Gwangju, Korea

    W. J. Jung, Y. L. Jin, R. D. Park & K. Y. Kim

  2. # 521 Molecular Biochemistry Laboratory & Biodefensive Substances Group, Chonnam National University, 500-757, Gwangju, Korea

    K. T. Lim

  3. Department of Animal Science, and Institute of Agricultural Science & Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju, P.O. Box 205, 500-757, Gwangju, Korea

    T. H. Kim

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  1. W. J. Jung
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  2. Y. L. Jin
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  3. R. D. Park
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  4. K. Y. Kim
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  5. K. T. Lim
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  6. T. H. Kim
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Correspondence to T. H. Kim.

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Jung, W.J., Jin, Y.L., Park, R.D. et al. Treatment of Paenibacillus illinoisensis suppresses the activities of antioxidative enzymes in pepper roots caused by Phytophthora capsici infection. World J Microbiol Biotechnol 22, 901–907 (2006). https://doi.org/10.1007/s11274-006-9131-7

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  • DOI: https://doi.org/10.1007/s11274-006-9131-7

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