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Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus

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An Erratum to this article was published on 25 October 2012

Abstract

A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 × 107–1.2 × 102 and from an average of 1.4 × 107–1.4 × 104 copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan® and SYBR® Green technologies provided accurate and reliable methods for monitoring, detection, discrimination, and quantification of WDV.

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Acknowledgments

This study was supported by grants from the Ministry of Agriculture, Czech Republic for the project Nos. MZE0002700604 (50%) and QH81269 (50%). The authors are deeply grateful to Dr. Laurence Garnier for his critical reading of the manuscript and valuable comments. The English of the manuscript was edited by International Science Editing http://www.internationalscienceediting.com.

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Authors and Affiliations

  1. Division of Plant Health, Department of Virology, Crop Research Institute, Prague, 16106, Czech Republic

    S. Gadiou, J. Ripl, B. Jaňourová, J. Jarošová & J. K. Kundu

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  1. S. Gadiou
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  2. J. Ripl
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  3. B. Jaňourová
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  4. J. Jarošová
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Correspondence to J. K. Kundu.

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Gadiou, S., Ripl, J., Jaňourová, B. et al. Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus . Virus Genes 44, 349–355 (2012). https://doi.org/10.1007/s11262-011-0699-0

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  • DOI: https://doi.org/10.1007/s11262-011-0699-0

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