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Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic

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Abstract

Cereal-infecting Mastreviruses are one of the most ubiquitous of viruses, having caused huge yield losses during the last decade in the Czech Republic. The presence of two strains of Wheat dwarf virus (WDV), one of which is wheat adapted and one barley adapted, have been confirmed from among field samples of wheat and barley plants. The virus typing was conducted by both PCR-RFLP and sequencing-based methods. The Czech WDV isolates of the barley strain are more variable than the isolates of the wheat strain, separating them into two clades, one representing a pool of divergent isolates. The RFLP analysis confirmed the separation of the Czech isolates into two strains and highlights a powerful method for the rapid diagnosis of both the wheat and barley strains. Additionally, both RFLP and sequence analysis have shown that the barley strain is restricted to the barley host, while the wheat strain is present in both wheat and barley plants.

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Acknowledgments

This work was supported by Project No. QH81269. We thank Mrs. Zuzana Červená for her excellent technical assistance. The authors are deeply grateful to Peter Lemkim and Dorothy Mogg for proofreading the English of the manuscript.

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Authors and Affiliations

  1. Department of Virology, Crop Research Institute, Prague, 161 06, Czech Republic

    J. K. Kundu & S. Gadiou

  2. Division of Diagnostics, State Phytosanitary Administration, Šlechtitelů 23/773, Olomouc, 779 00, Czech Republic

    G. Červená

Authors
  1. J. K. Kundu
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  2. S. Gadiou
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  3. G. Červená
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Correspondence to J. K. Kundu.

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J. K. Kundu and S. Gadiou have collaborated equally on this work.

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Kundu, J.K., Gadiou, S. & Červená, G. Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic. Virus Genes 38, 468–474 (2009). https://doi.org/10.1007/s11262-009-0352-3

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  • DOI: https://doi.org/10.1007/s11262-009-0352-3

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