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Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of Banana bunchy top virus originating from Pakistan

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Abstract

Five genes encoded by Banana bunchy top virus (BBTV) originating from Pakistan were expressed in Nicotiana benthamiana using a Potato virus X (PVX) vector. Expression of the master replication-associated protein (mRep) and movement protein (MP) resulted in necrotic cell death of inoculated tissues, as well as leaf curling and necrosis along the veins in newly emerging leaves. The systemic necrosis induced by the expression of MP was discolored (dark) in comparison to that induced by mRep. Expression of the cell-cycle link protein (Clink), the coat protein (CP), and the nuclear shuttle protein from the PVX vector induced somewhat milder symptoms, consisting of mild leaf curling and mosaic, although expression of the CP caused a necrotic response in inoculated leaf. The accumulation of viral RNA was enhanced by MP, Clink, and CP. Of the five BBTV-encoded gene products two, the MP and Clink, stabilized GFP-specific mRNA and reduced GFP-specific small interfering RNA in N. benthamiana line 16c when expressed under the control of the 35S promoter and co-inoculated with a construct for the expression of GFP hairpin RNA construct. These results identified MP and Clink as suppressors of RNA silencing. Taken together the ability of MP to induce severe symptoms in plants and suppress RNA silencing implicates this product as a major pathogenicity determinant of BBTV.

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Acknowledgments

M.I. was supported by a PhD fellowship from the Higher Education Commission (HEC), Government of Pakistan. R.W.B. is supported by the HEC under the “Foreign Faculty Hiring Program.” The authors are grateful to Dr. Peter Moffett (Boyce Thompson Institute for Plant Research) for providing the TCV-CP and HC-Pro constructs used in the study. The funds for this study were provided by a Pak-US linkage project.

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Authors and Affiliations

  1. Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan

    Imran Amin, Muhammad Ilyas, Javaria Qazi, Rohina Bashir, Shahid Mansoor & Rob W. Briddon

  2. ILTAB, Danforth Plant Science Center, 975 North Warson Road, St Louis, MO, 63132, USA

    Jitender S. Yadav & Claude M. Fauquet

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  1. Imran Amin
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Correspondence to Rob W. Briddon.

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Amin, I., Ilyas, M., Qazi, J. et al. Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of Banana bunchy top virus originating from Pakistan. Virus Genes 42, 272–281 (2011). https://doi.org/10.1007/s11262-010-0559-3

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  • DOI: https://doi.org/10.1007/s11262-010-0559-3

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