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Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts

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Abstract

Expression cassettes containing a duplicated cauliflower mosaic virus (CaMV) 35S promoter fused to a polylinker preceded by the CCACCATGG and AACAATGG sequences were constructed. These two sequences correspond to the consensus sequences around the translation start codons in vertebrates and plants respectively. Translational fusions were made with the β-glucuronidase-coding sequence and transient expression was recorded in tobacco mesophyll protoplasts. Approximately three times more GUS activity was found in protoplasts incubated with the constructs harbouring translational fusions as compared to a control harbouring a transcriptional fusion. No significant difference was observed between GUS activities obtained with the two consensus sequences.

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Authors and Affiliations

  1. Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, UK

    F. Guerineau

  2. John Innes Centre for Plant Science Research, John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    A. Lucy & P. Mullineaux

Authors
  1. F. Guerineau
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  2. A. Lucy
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  3. P. Mullineaux
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Guerineau, F., Lucy, A. & Mullineaux, P. Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts. Plant Mol Biol 18, 815–818 (1992). https://doi.org/10.1007/BF00020027

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  • DOI: https://doi.org/10.1007/BF00020027

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