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Effects of the 2b Protein of Cucumber mosaic virus Subgroup IB Strain IA on Different Transgene-Induced RNA Silencing Pathways

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Abstract

The Cucumber mosaic virus (CMV) 2b protein has multiple activities as a viral suppressor of RNA silencing (VSR). Here, we characterized the 2b protein (IA2b) of a CMV-IA isolate. This IA2b protein has two nuclear localization signals, and when it was fused with GFP, the protein was found to be preferentially localized in the nucleus. A transgenic tobacco line, 2b8, expressing the IA2b gene showed an enhanced expression of an agroinfiltrated sense transgene, which indicated that IA2b has VSR activity. When 2b8 plants were crossed with plants showing sense-transgene-induced post-transcriptional gene silencing (S-PTGS), the IA2b protein caused the disappearance of small interfering RNAs (siRNAs) and the S-PTGS phenotype was converted to the overexpression phenotype. When 2b8 plants were crossed with plants showing inverted repeat-induced post-transcriptional gene silencing (IR-PTGS), the IA2b protein did not suppress the accumulation of siRNAs processed from hairpin RNAs and the silencing phenotype was maintained. When a tobacco plant showing an overexpressed phenotype of a sense transgene was crossed with IR-PTGS plants targeting the same transgene, the primary siRNAs that were produced by the IR-PTGS construct triggered the production of the secondary siRNAs through the RNA-dependent RNA polymerase6 (RDR6)-dependent pathway. The IA2b protein suppressed the secondary siRNA formation but not the primary siRNA production from hairpin RNAs, resulting in the silencing phenotype from IR-PTGS being maintained. These results indicate that IA2b suppresses RDR6-dependent siRNA production and does not interfere with the silencing step by siRNA-guided RNA degradation.

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Acknowledgements

The authors are grateful to Drs. Keiko T. Natsuaki and Yasuo Niwa for the provision of the IA2b cDNA and sGFP clones, respectively.

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Authors and Affiliations

  1. Graduate School of Horticulture, Chiba University, 648 Matsudo, Chiba, 271-8510, Japan

    Mayuko Koizumi, Yuta Saeki, Sayaka Hirai, Shin-ichiro Oka & Hiroaki Kodama

  2. Graduate School of Science and Technology, 1-33 Yayoi-cho, Chiba, 263-8522, Japan

    Yumi Shimotori

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  1. Mayuko Koizumi
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  2. Yumi Shimotori
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  3. Yuta Saeki
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Correspondence to Hiroaki Kodama.

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Koizumi, M., Shimotori, Y., Saeki, Y. et al. Effects of the 2b Protein of Cucumber mosaic virus Subgroup IB Strain IA on Different Transgene-Induced RNA Silencing Pathways. Plant Mol Biol Rep 35, 265–272 (2017). https://doi.org/10.1007/s11105-016-1020-0

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