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Carboxyl terminus heterogeneity of type IV fimbrial subunit protein of Pasteurella multocida isolates

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Abstract

Pasteurella multocida, a Gram-negative bacterial pathogen, known to affect a wide range of domestic as well as wild animal and avian species throughout the world by causing either systemic or localized infections termed as ‘pasteurellosis’. P. multocida isolates are known to possess type IV fimbriae (pili) as one of the major virulence factors based on their role in adhesion to host surfaces and subsequent pathogenesis. In the present study, ptfA gene of Indian P. multocida isolates (n = 8) originated from different animal (buffalo, sheep, goat, pig) and avian host species (chicken, turkey, duck, quail) were amplified, cloned, sequenced and compared with available ptfA/fimbrial protein sequences in GenBank/publications (n = 22) to understand its variability with respect to geography/host/serogroup/disease specific patterns. Multiple sequence alignment revealed highly conserved N-terminus α-1 helix region and heterogeneous C-terminus (68–137 aa) comprised of β-strand regions (β1, β2, β3, β4) with conserved two pairs of cysteine residues. Interestingly, an existence of absolute homogeneity among the P. multocida isolates that caused haemorrhagic septicaemia in bovines and septicaemic pasteurellosis in sheep and goats was noticed. Pig isolates had 99.3 % homogeneity. On contrary, more diversity (35.8 %) was observed among isolates that caused fowl cholera in avians irrespective of identical capsular/somatic serogroup and similar host species. Phylogenetic analysis based on nucleotide sequences of ptfA gene revealed formation of mixed clusters with isolates representing different disease conditions as well as serogroups irrespective of country of origin which indicated the possible role of cross-species transmission among different animal/avian species. The study indicated highly conserved and host specific fimbriae among animal species than relatively divergent fimbriae among avian species.

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References

  • Adler B, Bulach D, Chung J, Doughty S, Hunt M, Rajakumar K, Serrano M, Zanden A, Zhang Y, Ruffolo C (1999) Candidate vaccine antigens and genes in Pasteurella multocida. J Biotechnol 73:83–90

    Article  PubMed  CAS  Google Scholar 

  • Doughty SW, Ruffalo CG, Adler B (2000) The type 4 fimbrial subunit gene of Pasteurella multocida. Vet Microbiol 72:79–90

    Article  PubMed  CAS  Google Scholar 

  • Glorioso JC, Jones GW, Rush HG, Pentler LJ, Darif CA, Coward JE (1982) Adhesion of type A Pasteurella multocida to rabbit pharyngeal cells and its possible role in rabbit respiratory tract infections. Infect Immun 35:1103–1109

    PubMed  CAS  Google Scholar 

  • Harper M, Boyce JD, Adler B (2006) Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol Lett 265:1–10

    Article  PubMed  CAS  Google Scholar 

  • Hatfaludi T, Al-Hasani K, Boyce JD, Adler B (2010) Outer membrane proteins of Pasteurella multocida. Vet Microbiol 144:1–17

    Article  PubMed  CAS  Google Scholar 

  • Hunt ML, Adler B, Townsend KM (2000) The molecular biology of Pasteurella multocida. Vet Microbiol 72:3–25

    Article  PubMed  CAS  Google Scholar 

  • Johnson TJ, Abrahante JE, Hunter SS, Hauglund M, Tatum FM, Maheswaran SK, Briggs RE (2013) Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity. BMC Microbiol 13:106

    Article  PubMed  Google Scholar 

  • Kumar AA, Shivachandra SB, Biswas A, Singh VP, Singh VP, Srivastava SK (2004) Prevalent serotypes of Pasteurella multocida isolated from different animal and avian species in India. Vet Res Commun 28:657–667

    Article  PubMed  CAS  Google Scholar 

  • Liu W, Yang M, Xu Z, Zheng H, Liang W, Zhou R, Wu B, Chen H (2012) Complete genome sequence of Pasteurella multocida HN06, a toxigenic strain of serogroup D. J Bacteriol 194:3292–3293

    Article  PubMed  CAS  Google Scholar 

  • May BJ, Zhang Q, Li LL, Paustian ML, Whittam TS, Kapur V (2001) Complete genomic sequence of Pasteurella multocida, Pm70. Proc Natl Acad Sci U S A 98:3460–3465

    Article  PubMed  CAS  Google Scholar 

  • Michael GB, Kadlec K, Sweeney MT, Brzuszkiewicz E, Liesegang H, Daniel R, Murray RW, Watts JL, Schwarz S (2012) ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer. J Antimicrob Chemother 67:91–100

    Article  PubMed  CAS  Google Scholar 

  • Myint A, Carter GR (1989) Prevention of haemorrhagic septicaemia in cattle and buffaloes with a live vaccine. Vet Rec 124:508–509

    Article  PubMed  CAS  Google Scholar 

  • Patel P, Marrs FC, Mattick J, Ruehl S, Taylor WW, Koomey M (1991) Shared antigenicity and immunogenicity of type 4 pilins expressed by Pseudomonas aeruginosa, Moraxilla bovis, Neisseria gonorrhoeae, Dichelobacternodosus and Vibriocholerae. Infect Immun 59:4674–4677

    PubMed  CAS  Google Scholar 

  • Ruffalo GC, Carmel G, Tennet JM, Michalski W, Adler B (1997) Identification, purification and characterization of type 4 fimbriae of Pasteurella multocida. Infect Immun 65:339–343

    Google Scholar 

  • Sellyei B, Bányai K, Magyar T (2010) Characterization of the ptfA gene of avian Pasteurella multocida strains by allele-specific polymerase chain reaction. J Vet Diagn Investig 22:607–610

    Article  Google Scholar 

  • Shivachandra SB, Viswas KN, Kumar AA (2011) A review of haemorrhagic septicaemia in cattle and buffalo. Anim Heal Res Rev 12:67–82

    Article  CAS  Google Scholar 

  • Shivachandra SB, Yogisharadya R, Ahuja A, Bhanuprakash V (2012) Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli. Res Vet Sci 93:1128–1131

    Article  PubMed  CAS  Google Scholar 

  • Siju J, Kumar AA, Shivachandra SB, Chaudhuri P, Srivastava SK, Singh VP (2007) Cloning and characterization of type 4 fimbrial gene (ptfA) of Pasteurella multocida serogroup B:2 (strain P52). Vet Res Commun 31:397–404

    Article  PubMed  CAS  Google Scholar 

  • Strom MS, Lory S (1993) Structure-function and biogenesis of the type IV pili. Annu Rev Microbiol 47:565–596

    Article  PubMed  CAS  Google Scholar 

  • Tomer P, Chaturvedi GC, Monga P (2004) Detection of fimbriae on haemorrhagic septicaemia associated Pasteurella multocida B:2 isolates. Indian J Anim Sci 74:1199–1201

    Google Scholar 

  • Verma R, Jaiswal TN (1998) Haemorrhagic septicaemia vaccines. Vaccine 16:1184–1190

    Article  PubMed  CAS  Google Scholar 

  • Wizemann TM, Adamou JE, Langermann S (1999) Adhesins as targets for vaccine development. Emerg Infect Dis 5:395–403

    Article  PubMed  CAS  Google Scholar 

  • Yasin IS, Mohd Yusoff S, Mohd ZS, Abd Wahid Mohd E (2011) Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats. Trop Anim Heal Prod 43:179–187

    Article  Google Scholar 

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Acknowledgments

Authors are thankful to the Director, Indian Veterinary Research Institute (IVRI), Izatnagar; the Indian Council of Agricultural Research (ICAR), New Delhi; for providing the necessary facilities and Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, New Delhi, for providing financial assistance (DBT Grant No.BT/PR15174/GBD/27/357/2011) to carry out the research on P. multocida.

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Authors and Affiliations

  1. Clinical Bacteriology Laboratory (CBL), Indian Veterinary Research Institute (IVRI), Regional Campus, Mukteswar, 263138, Nainital (District),, Uttarakhand (UK), India

    Sathish Bhadravati Shivachandra & Abhinendra Kumar

  2. Division of Bacteriology and Mycology, Indian Veterinary Research Institute (IVRI), Izatnagar, 243122, Uttar Pradesh, India

    K. N. Viswas

  3. Division of Virology, Indian Veterinary Research Institute (IVRI), Regional Campus, Mukteswar, 263138, Nainital (District),, Uttarakhand (UK), India

    Revanaiah Yogisharadhya & M. A. Ramakrishnan

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  1. Sathish Bhadravati Shivachandra
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  2. Abhinendra Kumar
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  3. Revanaiah Yogisharadhya
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  4. M. A. Ramakrishnan
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  5. K. N. Viswas
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Correspondence to Sathish Bhadravati Shivachandra.

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Shivachandra, S.B., Kumar, A., Yogisharadhya, R. et al. Carboxyl terminus heterogeneity of type IV fimbrial subunit protein of Pasteurella multocida isolates. Vet Res Commun 37, 269–275 (2013). https://doi.org/10.1007/s11259-013-9569-7

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  • DOI: https://doi.org/10.1007/s11259-013-9569-7

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