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Phylogenetic Analysis Based on OmpA Protein Sequences of Diverse Pasteurella multocida Strains Originated from Different Animal Host Species

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Pasteurella multocida causes endemic diseases of economic importance in livestock. A number of capsular serotypes of P. multocida bacteria are associated with different clinical diseases in the host species. Outer membrane proteins (OMPs) are at the interface of bacterium and host which are likely to play an important roles in host specificity and disease. The prime objective of the present study was to comparatively analyze seven P. multocida strains obtained from sheep, buffaloes, rabbit and pigs succumbed to different clinical conditions by ompA gene sequence analysis in conjunction with the capsular type. The ompA genes of various capsular serotypes from host species origins were amplified and sequenced. Further, sequence, phylogenetic and structural analyses were carried out. Analysis revealed that length of CDs region in different serogroups of P. multocida varied from ~ 1050 to 1077 bp, being highest in serogroups B (1077 bp) and lowest in serogroups A of rabbit strain (1050 bp). Nucleotide length was same in all sheep serogroup A strains (1059 bp). Protein domain analysis of OmpA showed signal peptide, N-terminal ompA domain and C-terminal ligand binding domain. The phylogenetic analysis showed two major clusters of the bovine and porcine/ sheep/rabbit/human strains, which was further divided in to different sub clusters such as subcluster I = NIVEDIPm sheep strains serogroup A, subcluster II = NIVEDIPm rabbit strains serogroup A, subcluster III = NIVEDIPm pig strains serogroup A, subcluster IV = NIVEDIPm pig strains serogroup D, subcluster V = NIVEDIPm bovine strains serogroup B. This study concluded that the clustering of P. multocida based on ompA proteins was associated with capsular serogroup and host species.

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Data Availability

ompA gene sequence of strains (NIVEDIPm 2, 17, 19, 29, 34, 40, 41) was deposited in NCBI database (GenBank), and accession number was assigned MW065783, MW065784, MW065785, MW065786, MW065787, MW065788 and MW065789, respectively.

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Acknowledgements

Authors are thankful to the Indian Council of Agricultural Research (ICAR), New Delhi, and ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, Karnataka, India, for providing the necessary facilities and financial assistance to carry out the research on P. multocida. This study was a part of doctoral research carried out by AP under the institute funded project (Code: IXX12176) on ‘Epidemiology of Haemorrahgic septicaemia (HS) in India’ granted to SBS.

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Authors and Affiliations

  1. ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Yelahanka, Bengaluru, Karnataka, 560064, India

    Awadhesh Prajapati, Revanaiah Yogisharadhya, Mohammed Mudassar Chanda, Suresh Kumar Mendem & Sathish Bhadravati Shivachandra

  2. CCS-National Institute of Animal Health (NIAH), Baghpat, Uttar Pradesh (UP), 250609, India

    Nihar Nalini Mohanty

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  1. Awadhesh Prajapati
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  2. Revanaiah Yogisharadhya
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  3. Mohammed Mudassar Chanda
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  5. Suresh Kumar Mendem
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  6. Sathish Bhadravati Shivachandra
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Correspondence to Sathish Bhadravati Shivachandra.

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Significance Statement

Comparatively analyzed seven P. multocida isolates obtained from sheep, buffaloes, rabbit and pigs succumbed to different clinical conditions by ompA gene sequence-based phylogenetic analysis in conjunction with the capsular types which indicated two distinct clusters.

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Prajapati, A., Yogisharadhya, R., Chanda, M.M. et al. Phylogenetic Analysis Based on OmpA Protein Sequences of Diverse Pasteurella multocida Strains Originated from Different Animal Host Species. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 94, 439–447 (2024). https://doi.org/10.1007/s40011-023-01543-7

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