Skip to main content
Log in

Cloning and Characterization of Type 4 Fimbrial Gene (ptfA) of Pasteurella multocida Serogroup B:2 (Strain P52)

  • Short Communication
  • Published:
Veterinary Research Communications Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Abbreviations

HS:

haemorrahagic septicaemia

PCR:

polymerase chain reaction

PtfA:

Pasteurella multocida type 4 fimbrial protein A

dNTP:

deoxynucleotide triphosphate

SDS-PAGE:

sodium dodecyl sulphate-polyacrylamide gel electrophoresis

References

  • Bjerrum, O.J. and Schafter-Nilson, C., 1986. Buffer system and transfer parameters for semidry electro blotting with a horizontal apparatus.In: Dunn (ed.), Electrophoresis 86, (VCH, Weinheim), 315–327

    Google Scholar 

  • Brumell, H.J. and Finlay, B.B., 2000. Bacterial adherence, colonization and invasion of mucosal surfaces. In: K.A. Brogden, J.A. Roth, T.B. Stanton, C.A. Bolin, F.C. Minion and M.J. Wannemue (eds), Virulence Mechanisms of Bacterial Pathogens, 3rd edn (ASM press, Washington DC), 3–17

    Google Scholar 

  • Dabo, S.M., Confer, A.W. and Quijano-Blas, R.A., 2003. Molecular and immunological characterization of Pasteurella multocida serotype A:3 OmpA: evidence of its role in P. multocida interaction with extracellular matrix molecules. Microbial Pathogenesis, 35, 147–157

    Article  PubMed  CAS  Google Scholar 

  • Doughty, W.S., Ruffalo, G.C. and Alder, B., 2000. The type 4 fimbrial subunit gene of Pasteurella multocida. Veterinary Microbiology, 72, 79–90

    Article  PubMed  CAS  Google Scholar 

  • Elleman, T.C., Hoyne P.A., Stewart, D.J., McKern, N.M. and Peterson, J.E., 1986. Expression of Pili from Bacteroides nodosus in Pseudomonas aeruginosa. Journal of Bacteriology, 168, 574–580

    PubMed  CAS  Google Scholar 

  • Finlay, R.B. and Falkow, S., 1989. Common themes in microbial pathogenicity. Microbiological Reviews, 53, 210–230

    PubMed  CAS  Google Scholar 

  • Glorioso, J.C., Jones, G.W., Rush, H.G., Pentler, L.J., Darif, C.A. and Coward, J.E., 1982. Adhesion of type A Pasteurella multocida to rabbit pharyngeal cells and its possible role in rabbit respiratory tract infections. Infection and Immunity, 35, 1103–1109

    PubMed  CAS  Google Scholar 

  • Heckels, J.E., 1989. Structure and function of pili of pathogenic Neisseria species. Clinical Microbiology Reviews, 2, 66–S73

    Google Scholar 

  • Hunt, M.L., Adler, B. and Townsend, K.M., 2000. The molecular biology of Pasteurella multocida. Veterinary Microbiology, 72, 3–25

    Article  PubMed  CAS  Google Scholar 

  • Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 277, 680–685.

    Article  Google Scholar 

  • Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J., 1951. Protein measurement with Folin-phenol reagent. Journal of Biological Chemistry, 179, 265–271

    Google Scholar 

  • Mizhan, S., Henk, A., Stallings, A., Maier, M. and Lee, D.M., 2000. Cloning and characterization of sialidases with 2′-6′ and 2–3′sialyl Lactose specificity from Pasteurella multocida. Journal of Bacteriology, 182, 6874–6883

    Article  Google Scholar 

  • Moxon, E.R. and Kroll, J.S., 1990. The role of bacterial polysaccharide capsules as virulence factors. Current Topics on Microbiology and Immunology, 150, 65–85

    CAS  Google Scholar 

  • Murphy, G. and Kavanagh, T., 1988. Speeding up the sequencing of double stranded DNA. Nucleic Acids Research, 16, 5178

    Article  Google Scholar 

  • Patel, P., Marrs, F.C., Mattick, J., Ruehl, S., Taylor, W.W. and Koomey, M., 1991. Shared antigenicity and immunogenicity of type 4 pilins expressed by Pseudomonas aeruginosa, Moraxilla bovis, Neisseria gonorrhoeae, Dichelobacternodosus and Vibriocholerae. Infection and Immunity, 59, 4674–4677

    PubMed  CAS  Google Scholar 

  • Ruffalo, G.C., Carmel, G., Tennet, J.M., Michalski, W. and Adler, B., 1997. Identification, purification and characterization of type 4 fimbriae of Pasteurella multocida. Infection and Immunity, 65, 339–343

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd edn, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY)

    Google Scholar 

  • Singh, V.P., Kumar, A.A., Srivastava, S.K. and Rathore, B.S., 1996. Significance of HS in Asia: India. International Workshop on Diagnosis and Control of HS, 28–30 May, Bali, Indonesia

  • Verma, R. and Jaiswal, T.N., 1998. Haemorrhagic septicaemia vaccines. Vaccine, 16, 1184–1190

    Article  PubMed  CAS  Google Scholar 

  • Virji, M., Saunders, G., Sims, K., Makepeace, D., Maskell, J. and Ferguson, D.J., 1993. Pilus facilitated adherence of Neisseria meningitides to human epithelial and endothelial cells: modulation of adherence phenotype occurs concurrently with changes in primary amino acid sequence and the glycosylation status of pilin. Molecular Microbiology, 10, 1013–1028

    Article  PubMed  CAS  Google Scholar 

  • Wilson, K., 1987. Preparation of genomic DNA from bacteria. In: F.M. Ausubal, R. Brent, R.L. Kirston, D.D., Moore, J.G. Seidman, J.A. Smith and K. Struhe (eds), Current Protocols in Molecular Biology, vol. 1, (Wiley, New York) 2.4.1–2.4.2

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. A. Kumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siju, J., Kumar, A.A., Shivachandra, S.B. et al. Cloning and Characterization of Type 4 Fimbrial Gene (ptfA) of Pasteurella multocida Serogroup B:2 (Strain P52). Vet Res Commun 31, 397–404 (2007). https://doi.org/10.1007/s11259-006-3372-7

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11259-006-3372-7

Keywords

Navigation