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Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells

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Abstract

The aim of the study was to compare in vitro the stemness features of horse progenitor cells derived from bone marrow (BM-MSCs), amniotic fluid (AF-MSCs) and umbilical cord matrix (EUC-MSCs). It has been suggested that there may be a stem cell population within both umbilical cord matrix and amniotic fluid. However, little knowledge exists about the characteristics of these progenitor cells within these sources in the equine species. This study wanted to investigate an alternative and non-invasive stem cell source for the equine tissue engineering and to learn more about the properties of these cells for future cell banking. Bone marrow, umbilical cord and amniotic fluid samples were harvested from different horses. Cells were analyzed for proliferation, immunocytochemical, stem cell gene expression and multilineage plasticity. BM- and AF-MSCs took similar time to reach confluence and showed comparable plating efficiency. All cell lines expressed identical stem cell markers and capability to differentiate towards osteogenic lineage. Almost all cell lines differentiated into the adipogenic lineage as demonstrated by cytochemical staining, even if no adipose gene expression was detectable for AF-MSCs. AF- and EUC-MSCs showed a limited chondrogenic differentiation compared with BM-MSCs as demonstrated by histological and biochemical analyses. These findings suggest that AF-MSCs appeared to be a readily obtainable and highly proliferative cell line from an uninvasive source that may represent a good model system for stem cell biology. More studies are needed to investigate their multilineage potential. EUC-MSCs need to be further investigated regarding their particular behavior in vitro represented by spheroid formation.

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Acknowledgments

The authors thank the Filarete Foundation and Galeazzi Orthopaedic Institute for financial support of this project and technical help with histology and chondrogenic differentiation analyses, respectively. B. Corradetti PhD fellowship is partially supported by a research grant from Università Politecnica delle Marche to Professor D. Bizzaro.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Veterinary Clinical Science, Equine Reproduction Unit, Università degli Studi di Milano, Strada dell’Università 6, Località Polledra, 26900, Lodi, Italy

    Arianna Barbara Lovati, Anna Lange Consiglio & Fausto Cremonesi

  2. Department of Biochemistry, Biology and Genetics, Università Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy

    Bruna Corradetti & Davide Bizzaro

  3. IRCCS Istituto Ortopedico Galeazzi, Cell and Tissue Engineering Laboratory, via R. Galeazzi 4, 20161, Milan, Italy

    Elisa Bonacina

  4. Mouse & Animal Pathology Laboratory (MAPLab)—FONDAZIONE FILARETE Viale Ortles 22/4, 20139, Milano (MI), Italy

    Camilla Recordati

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  1. Arianna Barbara Lovati
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  2. Bruna Corradetti
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Correspondence to Arianna Barbara Lovati.

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Fig. 4

Immunostaining characterization of BM-, AF and EUC-MSCs. Cells were stained just with the secondary antibodies to evaluate their negative response (Magnification: 20X; scale bars: 20 μm) (TIFF 6008 kb)

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Lovati, A.B., Corradetti, B., Lange Consiglio, A. et al. Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells. Vet Res Commun 35, 103–121 (2011). https://doi.org/10.1007/s11259-010-9457-3

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  • DOI: https://doi.org/10.1007/s11259-010-9457-3

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