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Biological characterization and pluripotent identification of ovine amniotic fluid stem cells

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Abstract

Mesenchymal stem cells derived from amniotic fluid have become one of the most potential stem cell source for cell-based therapy for the reason they can be harvested at low cost and without ethical problems. Here, we obtained amniotic fluid stem cells (AFSCs) from ovine amniotic fluid and studied the expansion capacity, cell markers expression, karyotype, and multilineage differentiation ability. In our work, AFSCs were subcultured to passage 62. The cell markers, CD29, CD44, CD73 and OCT4 which analyzed by RT-PCR were positive; CD44, CD73, CD90, CD105, NANOG, OCT4 analyzed by immunofluorescence and flow cytometry were also positive. The growth curves of different passages were all typically sigmoidal. The different passages cells took on a normal karyotype. In addition, AFSCs were successfully induced to differentiate into adipocytes, osteoblasts and chondrocytes. The results suggested that the AFSCs isolated from ovine maintained normal biological characteristics and their multilineage differentiation potential provides many potential applications in cell-based therapies and tissue engineering.

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Acknowledgements

This research was supported by the Project supported by the National Natural Science Foundation of China (Grant Nos. 31472099, 31672404).

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Author notes

  1. Wenhua Pei and Tengfei Lu have contributed equally to this work.

Authors and Affiliations

  1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanmingyuan West road, haidian district, Beijing, 100193, China

    Wenhua Pei, Tengfei Lu, Meng Ji, Xiangchen Li & Weijun Guan

  2. College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China

    Kunfu Wang

  3. Research Center for Sports Scientific Experiment, Harbin Sport University, Harbin, People’s Republic of China

    Shuang Zhang

  4. College of Human Movement Science, Harbin Sport University, Harbin, 150040, China

    Fenghao Chen

  5. College of Animal Science and Technology, Agricultural University of Hebei, Baoding, 071000, China

    Lu Li

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  1. Wenhua Pei
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  2. Tengfei Lu
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  3. Kunfu Wang
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  4. Meng Ji
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  5. Shuang Zhang
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  6. Fenghao Chen
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  7. Lu Li
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  8. Xiangchen Li
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  9. Weijun Guan
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Correspondence to Xiangchen Li or Weijun Guan.

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Pei, W., Lu, T., Wang, K. et al. Biological characterization and pluripotent identification of ovine amniotic fluid stem cells. Cytotechnology 70, 1009–1021 (2018). https://doi.org/10.1007/s10616-017-0115-2

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  • DOI: https://doi.org/10.1007/s10616-017-0115-2

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