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Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses

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Abstract

In plants, nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes. In this study, we developed transgenic potato plants (Solanum tuberosum L. cv. Atlantic) expressing Arabidopsis NDPK2 (AtNDPK2) gene in cytosols under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SN plants) or enhanced CaMV 35S promoter (EN plants) and evaluated their tolerance to various environmental stress, including methyl viologen (MV)-mediated oxidative stress, high temperature, and salt stress. When 250 μM MV was sprayed to whole plants, plants expressing NDPK2 showed significantly an enhanced tolerance compared to non-transgenic (NT) plants. SN plants and EN plants showed 51% and 32% less visible damage than NT plants, respectively. Transcript level of AtNDPK2 gene and NDPK2 activity in SN plants following MV treatment well reflected the plant phenotype. Ascorbate peroxidase (APX) activity was also increased in MV-treated SN plants. In addition, SN plants showed enhanced tolerance to high temperature at 42°C. The photosynthetic activity of SN plants after treatment of high temperature was decreased by about 10% compared to the plants grown at 25°C, whereas that of NT plants declined by 30%. When treated with 80 mM NaCl onto the plantlets, both SN plants and EN plants also showed a significant reduced damage in root growth. These results indicate that overexpression of NDPK2 under the stress-inducible SWPA2 promoter might efficiently regulate the oxidative stress derived from various environmental stresses.

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Acknowledgements

This research was supported by grants from BioGreen21 Program (code #20070301034015), Rural Development Administration, Korea, from the Environmental Biotechnology National Core Research Center, KOSEF/MOST, Korea, and from the Korea Foundation for International Cooperation of Science and Technology (KICOS).

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Author notes

  1. Li Tang

    Present address: Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin, 300134, China

Authors and Affiliations

  1. Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon, 305–806, Korea

    Li Tang, Myoung Duck Kim, Kyoung-Sil Yang, Suk-Yoon Kwon, Sang-Soo Kwak & Haeng-Soon Lee

  2. Department of Environmental Horticulture, University of Seoul, Seoul, 130–743, Korea

    Sun-Hyung Kim

  3. Biofunction Research Team, Bioorganic Science Division, Korea Research Institute of Chemical Technology (KRICT), P.O. Box 107, Yuseong, Daejeon, 305–600, Korea

    Jin-Seog Kim

  4. Division of Applied Life Science, and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660–701, Korea

    Dae-Jin Yun

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  1. Li Tang
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Correspondence to Haeng-Soon Lee.

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Tang, L., Kim, M.D., Yang, KS. et al. Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses. Transgenic Res 17, 705–715 (2008). https://doi.org/10.1007/s11248-007-9155-2

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  • DOI: https://doi.org/10.1007/s11248-007-9155-2

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