Abstract
Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic potato plants with enhanced tolerance to environmental stress, the genes of both Cu/Zn superoxide dismutase and ascorbate peroxidase were expressed in chloroplasts under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SSA plants). SSA plants showed enhanced tolerance to 250 μM methyl viologen, and visible damage in SSA plants was one-fourth that of non-transgenic (NT) plants that were almost destroyed. In addition, when SSA plants were treated with a high temperature of 42°C for 20 h, the photosynthetic activity of SSA plants decreased by only 6%, whereas that of NT plants decreased by 29%. These results suggest that the manipulation of the antioxidative mechanism of the chloroplasts may be applied in the development of industrial transgenic crop plants with increased tolerance to multiple environmental stresses.
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References
Allen RD, Webb RP, Schake SA (1997) Use of transgenic plants to study antioxidant defenses. Free Radic Biol Med 23:473–479
Aono M, Saji H, Sakamoto A, Tanaka K, Kondo N (1995) Paraquat tolerance of transgenic Nicotiana tabacum with enhanced activities of glutathione reductase and superoxide dismutase. Plant Cell Physiol 36:1687–1691
Asada K (1999) The water–water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50:601–639
Badawi GH, Kawano N, Yamauchi Y, Shimada E, Sasaki R, Kubo A, Tanaka K (2004) Over-expression of ascorbate peroxidase in tobacco chloroplasts enhances the tolerance to salt stress and water deficit. Physiol Plant 121:231–238
Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795
Foyer CH, Descourvierse P, Kunert KJ (1994) Protection against oxygen radicals: an important defense mechanism studied in transgenic plants. Plant Cell Environ 17:507–523
Gong M, Li Y, Chen S (1998) Abscisic acid-induced thermotolerance in maize seedling is mediated by calcium and associated with antioxidant systems. J Plant Physiol 153:488–496
Jang HH, Lee KO, Chi YH, Jung BG, Park JH, Lee JR, Moon JC, Yun JW, Choi YO, Kim WY, Kang JS, Cheong GW, Yun DJ, Rhee SG, Cho MJ, Lee SY (2004) Two enzymes in one; two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecules chaperone function. Cell 28:625–635
Jeong MJ, Park SC, Byun MO (2001) Improvement of salt tolerance in transgenic potato plants by glyceraldehydes-3 phosphate dehydrogenase gene transfer. Mol Cells 12:185–189
Kim KY, Hur KH, Lee HS, Kwon SY, Hur Y, Kwak SS (1999) Molecular characterization of two anionic peroxidase cDNAs isolated from suspension cultures of sweetpotato. Mol Gen Genet 261:941–947
Kim KY, Kwon SY, Lee HS, Hur Y, Bang JW, Kwak SS (2003) A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. Plant Mol Biol 51:831–838
Kim SH, Hamada T (2005) Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L.). Lam Biotech Lett 27:1841–1845
Kwak SS, Kim SK, Lee MS, Jung KH, Park IH, Liu JR (1995) Three acidic peroxidases from suspension-cultures of sweetpotato. Phytochemistry 39:981–984
Kwon SY, Jeong YZ, Lee HS, Kim JS, Cho KY, Allen RD, Kwak SS (2002) Enhanced tolerance of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress. Plant Cell Environ 25:873–882
Lee HS, Kim KY, You SH, Kwon SY, Kwak SS (1999) Molecular characterization and expression of a cDNA encoding copper/zinc superoxide dismutase from cultured cells of cassava (Manihot esculenta Crantz). Mol Gen Genet 262:807–814
Moon H, Lee B, Choi G, Shin D, Prasad DT, Lee OS, Kwak SS, Kim DH, Nam JS, Bark J, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc Natl Acad Sci USA 100:358–363
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Ann Rev Plant Physiol Plant Mol Biol 49:249–279
Panchuk, II, Volkov RA, Schoffl F (2002) Heat stress- and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis. Plant Physiol 129:838–853
Payton P, Allen R, Trolinder N, Holaday A (1997) Over-expression of chloroplast-targeted Mn Superoxide dismutase in cotton does not alter the reduction of photosythesis after short exposures to low temperature and high light intensity. Photosynth Res 52:233–244
Payton P, Webb R, Kornyteyev D, Allen RD, Holaday AS (2001) Protecting cotton photosynthesis during moderate chilling at high light intensity by increasing chloroplastic antoxidant enzyme activity. J Exp Bot 52:2345–2354
Perl A, Perl-Treves R, Dalili S (1993) Enhanced oxidative stress defence in transgenic potato expressing Cu/Zn superoxide dismutase. Theor Appl Genet 85:568–576
Ross H (1986) Potato breeding: problems and perspectives. J Plant Breed Suppl 13. Adv Plant Breed. Parey, Berlin and Hamburg, 132 pp
Sen Gupta A, Heinen JL, Holaday AS, Burke JJ, Allen RD (1993) Increased resistance in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. Proc Natl Acad Sci USA 90:1629–1633
Storozhenko S, De Pauw P, Van Montagu M, Inze D, Kushnir S (1998) The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter. Plant Physiol 118:1005–1014
Tang L, Kwon SY, Kwak SS, Sung CK, Lee HS (2004) Selection of transgenic potato plants expressing both CuZnSOD and APX in chloroplasts with enhanced tolerance to oxidative stress. Korean J Plant Biotechnol 31:109–113
Tsang EW, Bowler C, Herouart D, Van Camp W, Villarroel R, Genetello C, Van Montagu M, Inze D (1991) Differential regulation of superoxide dismutases in plants exposed to environmental stress. Plant Cell 3:783–792
Van Camp W, Inze D, Van Montagu M (1997) The regulation and function of tobacco superoxide dismutases. Free Radic Biol Med 23:515–520
Yoshida K, Shinmyo A (2000) Transgene expression systems in plants, a natural bioreactor. J Biosci Bioeng 90:353–362
Acknowledgements
This work was supported by grants from the Biogreen 21 Program, Rural Development Administration, Korea, from the Environmental Biotechnology National Core Research Center, KOSEF/MOST, and from the International Collaboration Project, Ministry of Science and Technology (MOST), Korea.
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Communicated by I. S. Chung
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Tang, L., Kwon, SY., Kim, SH. et al. Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature. Plant Cell Rep 25, 1380–1386 (2006). https://doi.org/10.1007/s00299-006-0199-1
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DOI: https://doi.org/10.1007/s00299-006-0199-1