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Efficient and Stable Transformation of Lactuca sativa L. cv. Cisco (lettuce) Plastids

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Abstract

Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ~36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

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Authors and Affiliations

  1. Resarch Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizu-cho Soraku-gun, Kyoto, 619-0292, Japan

    Hirosuke Kanamoto, Satoru Okumura & Ken-Ichi Tomizawa

  2. Kitasato Institute for Life Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa, 228-8555, Japan

    Atsushi Yamashita, Hisabumi Takase & Masahira Hattori

  3. Nara Prefectural Agricultural Experiment Station, 88 Shijyo-cho, Kashihara, Nara, 634-0813, Japan

    Hiroshi Asao

  4. Graduate School of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192, Japan

    Akiho Yokota

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  1. Hirosuke Kanamoto
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  2. Atsushi Yamashita
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  3. Hiroshi Asao
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  4. Satoru Okumura
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  5. Hisabumi Takase
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  6. Masahira Hattori
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  7. Akiho Yokota
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  8. Ken-Ichi Tomizawa
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Correspondence to Hirosuke Kanamoto.

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Kanamoto, H., Yamashita, A., Asao, H. et al. Efficient and Stable Transformation of Lactuca sativa L. cv. Cisco (lettuce) Plastids. Transgenic Res 15, 205–217 (2006). https://doi.org/10.1007/s11248-005-3997-2

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  • DOI: https://doi.org/10.1007/s11248-005-3997-2

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