Abstract
As a model plant, poplar 84 K (Populus alba × P. glandulosa) plays a key role in fundamental research in forest molecular biology. Poplar 84 K is a suitable plant for in vitro polyploidy induction and to study the consequent trait variation among perennial trees. In this study, the results indicated that the concentration of NAA (naphthaleneacetic acid) and BA (benzyladenine) has substantial effects on the differentiation rate of adventitious shoots, and media consisting of 0.5 mg L−1 BA and 0.05 mg L−1 NAA yielded the greatest number of shoots per explant. A total of 98 tetraploids were successfully obtained by colchicine treatment on diploid leaves in vitro. The rate of tetraploids was substantially affected by pre-culture duration, colchicine concentration and exposure duration. The highest tetraploid induction efficiency was 37.03%, which was achieved by treating leaves with 50 mg L−1 colchicine for 3 days after 4 days of pre-culture. With increasing ploidy, obvious morphological differences were discovered between tetraploids and diploids. Compared with the diploids, the tetraploids had larger and thicker leaves, larger but sparser stomata, fewer and shorter roots, and larger protoplasts. These tetraploids serve to enrich the polyploid germplasm resources of Populus. Moreover, our study lays an important groundwork for polyploid gene function and is important for exploring trait variation among Populus trees with different ploidy levels.
Key message
In vitro induction of tetraploid by colchicine treatment and differences in morphological features between tetraploid and diploid plants.
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Abbreviations
- MS:
-
Murashige and Skoog (1962)
- BA:
-
Benzyladenine
- NAA:
-
1-Naphthaleneacetic acid
- IBA:
-
Indole butyric acid
- DAPI:
-
4,6-Diamidino-2-phenylindole
- MOPS:
-
4-Morpholinepropane sulfonate
- CPW:
-
Cell protoplast washing medium
- MES:
-
4-Morpholine ethane sulfonic acid
- BS:
-
Bovine serum albumin
- LSD:
-
Least significant difference
- SE:
-
Standard error
- SEM:
-
Scanning electron microscopy
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This work was financially supported by National Natural Science Foundation of China (31530012).
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XY and YY conceived and designed the research. YY conducted the experiments, analyzed data and wrote the manuscript. YC processed figures. All authors have read and agreed to the published version of the manuscript.
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Ren, Y., Jing, Y. & Kang, X. In vitro induction of tetraploid and resulting trait variation in Populus alba × Populus glandulosa clone 84 K. Plant Cell Tiss Organ Cult 146, 285–296 (2021). https://doi.org/10.1007/s11240-021-02068-5
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DOI: https://doi.org/10.1007/s11240-021-02068-5