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In vitro induction of polyploidy in japanese quince (Chaenomeles japonica)

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Abstract

In vitro conditions for Japanese quince polyploidisation were investigated. Microshoots and isolated cotyledons were treated with colchicine and oryzalin. Morphogenesis was more dependent on the concentration of colchicine or oryzalin than on the duration of exposure, genotype differences were observed. Low oryzalin concentrations had no impact on morphogenesis. Plants with changed chromosome numbers were obtained at 0.25–38 mM colchicine and 10–50 μM oryzalin concentrations. It was determined that stomata length is a suitable parameter for identifying putative Japanese quince tetraploids. Stomata of tetraploid shoots of the same clone were approximately 1/3 longer than in the diploids. It was shown that through polyploidisation gigas effect was not obtained in fruit size but tetraploids have reduced seed set and an increased proportion of fruit flesh.

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Abbreviations

BA:

6-benzylaminopurine

DMSO:

dimethyl sulfoxide

GA:

gibberellic acid

IBA:

indole-butyric acid

PPFD:

photosynthetic photon flux density

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Acknowledgements

This study was carried out with the financial assistance of the Commission of the European Communities, Agriculture and Fisheries (FAIR) specific RTD programme, CT 97–38–94, “Japanese quince (Chaenomeles japonica) – a new European fruit crop for production of juice, flavour and fibre”. It does not necessarily reflect its views and in no way anticipates the Commissions’ future policy in this area.

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Authors and Affiliations

  1. Lithuanian Institute of Horticulture, LT-5433, Babtai, Kaunas distr., Lithuania

    V. Stanys, G. Staniene & P. Duchovskis

  2. Department of Applied Biology, University of Helsinki, P.O. Box 27, FIN-00014, Helsinki, Finland

    A. Weckman

Authors
  1. V. Stanys
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  2. A. Weckman
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  3. G. Staniene
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  4. P. Duchovskis
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Correspondence to V. Stanys.

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Stanys, V., Weckman, A., Staniene, G. et al. In vitro induction of polyploidy in japanese quince (Chaenomeles japonica). Plant Cell Tiss Organ Cult 84, 263–268 (2006). https://doi.org/10.1007/s11240-005-9029-3

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  • DOI: https://doi.org/10.1007/s11240-005-9029-3

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