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Proteomic profile and polyamine contents are modulated by light source to promote in vitro shoot development in Cariniana legalis (Martius) O. Kuntze (Lecythidaceae)

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Abstract

The effect of different light sources on in vitro shoot development in Cariniana legalis, an endangered species from the Atlantic Forest, was evaluated. Cotyledonary and apical nodal explants were subjected to light-emitting diode (LED) lamps with different spectral combinations and fluorescent lamps (control). Shoot growth, endogenous contents of free polyamines (PAs) and proteomic profiles were analyzed at 60 days of development. Treatments consisting of white, low-blue and deep-red, with (W/lB/dR/fR) and without (W/lB/dR) far-red spectra, resulted in greater elongation of shoots from cotyledonary nodal explants, and the low-blue and deep-red spectral combination appeared to be a positive factor stimulating shade-avoidance responses. Shoots grown under the W/lB/dR LED exhibited greater elongation and higher contents of free putrescine, spermidine and total free PAs compared to those grown under the fluorescent lamp. Comparative proteomic analysis revealed 15 up- and 41 down-regulated proteins in shoots grown under the W/lB/dR LED lamp when compared to the control. The differentially up-regulated proteins in shoots grown under the LED lamp are related to cell organization and composition, as well as biological regulation processes, whereas proteins related to stress processes were down-regulated. The LED lamp consisting of white, low-blue and deep-red spectra increased shoot elongation in C. legalis, in association with differential accumulation of proteins and PAs, suggesting the relevance of source light on in vitro shoot development in this species.

Key message

A light source combining low-blue and deep-red spectra enhanced shoot elongation, differential protein accumulation and polyamine contents during in vitro shoot development in C. legalis.

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Abbreviations

LED:

Light-emitting diode

PAs:

Polyamines

PGRs:

Plant growth regulators

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

WPM:

Woody plant medium

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Acknowledgements

Funding for this work was provided by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (444,453/2014-8 and 307,596/2016-8) and the Fundação Carlos Chagas Filho de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ) (E26/010.001507/2014; E26/202.969/2016). This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001. VPMA and RSR acknowledge the scholarship funded by FAPERJ. JL is thankful for the scholarship provided by CAPES.

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Authors and Affiliations

  1. Laboratório de Biologia Celular e Tecidual (LBCT)-CBB, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ, CEP 28013-602, Brazil

    Joviana Lerin, Victor Paulo Mesquita Aragão & Claudete Santa-Catarina

  2. Laboratório de Biotecnologia (LBT)-CBB, UENF, Campos dos Goytacazes, RJ, Brazil

    Ricardo Souza Reis & Vanildo Silveira

  3. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil

    Ricardo Souza Reis & Vanildo Silveira

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  1. Joviana Lerin
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  2. Victor Paulo Mesquita Aragão
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  3. Ricardo Souza Reis
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  4. Vanildo Silveira
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  5. Claudete Santa-Catarina
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Contributions

CSC and JL conceived the study, designed the experiments and wrote the manuscript. JL was responsible for the in vitro cultures. JL, VPMA and CSC were responsible for the PA experiments and analyses. JL, RSR and VS were responsible for the proteomic analyses. All authors read and approved the final manuscript.

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Correspondence to Claudete Santa-Catarina.

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Lerin, J., Aragão, V.P.M., Reis, R.S. et al. Proteomic profile and polyamine contents are modulated by light source to promote in vitro shoot development in Cariniana legalis (Martius) O. Kuntze (Lecythidaceae). Plant Cell Tiss Organ Cult 137, 329–342 (2019). https://doi.org/10.1007/s11240-019-01574-x

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