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Kinsenoside and polysaccharide production by rhizome culture of Anoectochilus roxburghii in continuous immersion bioreactor systems

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Abstract

Anoectochilus roxburghii (Wall) Lindl. (Orchidaceae) is a precious raw material for medicine. However, the wild resource of A. roxburghii has been endangered, and artificial cultivation results in low yields. To provide rhizomes of A. roxburghii as alternative plant materials, the present study used continuous immersion bioreactor systems to investigate several factors affecting rhizome biomass and bioactive compound accumulation. The bioreactor with a net at the bottom of the sphere in the bioreactor was suitable for production of rhizomes. The rhizome biomass and kinsenoside and polysaccharide accumulation peaked at 30 days of the bioreactor culture. Thus, 30 days was the appropriate culture period. Maximum rhizome biomass and kinsenoside and polysaccharide accumulation were determined when a bioreactor was inoculated with 12.5 g L− 1 (fresh weight) of rhizomes, aerated at 500 mL min− 1, and maintained under 45 µmol m− 2 s− 1 light intensity. This process resulted in the production of 2980.5 mg L− 1 of kinsenoside and 5672.9 mg L−1 of polysaccharides.

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Acknowledgements

Project 31260182 and 31660080 supported by National Natural Science Foundation of China.

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Author notes

  1. Mei-Yu Jin and Lu Han have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, China

    Mei-Yu Jin, Lu Han, He Li, Hong-Qiu Wang, Xuan-Chun Piao & Mei-Lan Lian

Authors
  1. Mei-Yu Jin
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  2. Lu Han
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  3. He Li
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  4. Hong-Qiu Wang
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  5. Xuan-Chun Piao
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  6. Mei-Lan Lian
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Contributions

All authors contributed extensively to the work presented in this paper. MYJ and LH conducted bioreactor experiments, HL and HQW determined bioactive compound contents, XCP was responsible for viability tests and statistical analysis, and MLL designed experiments and wrote the paper.

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Correspondence to Mei-Lan Lian.

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Communicated by Nokwanda Pearl Makunga.

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Jin, MY., Han, L., Li, H. et al. Kinsenoside and polysaccharide production by rhizome culture of Anoectochilus roxburghii in continuous immersion bioreactor systems. Plant Cell Tiss Organ Cult 131, 527–535 (2017). https://doi.org/10.1007/s11240-017-1302-8

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  • DOI: https://doi.org/10.1007/s11240-017-1302-8

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