Abstract
To improve their growth and secondary metabolite production, we culturedMorinda citrifolia leaf cells for 3 weeks in bioreactors with different aeration volumes (0.05, 0.1, 0.2, or 0.3 vvm; or 0.05/0.1/0.2/0.3 vvm, as increased at 5-d interval), and photosynthetic photon fluxes (PPF; 0, 15, 30, or 45 μ,moL m-2 s-1). Cell growth was greatest (15.6 g L-1 dry weight) at 0.3 vvm whereas the accumulation of secondary metabolites (total anthraquinones, phenolics, and flavonoids) was maximized at 0.1 vvm. A PPF of 15 μmoLm-2 s-1 accelerated the accumulation of both cell biomass and metabolites. Dark-culturing suppressed cell growth, while a high PPF (45 μmoLm-2 s-1) inhibited metabolite biosynthesis. Further studies are required to understand the reason for differences in the effect of light on cell growth and secondary metabolite contents inM. citrifolia cell cultures.
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Ahmed, S., Hahn, EJ. & Paek, KY. Aeration volume and photosynthetic photon flux affect cell growth and secondary metabolite contents in bioreactor cultures ofMorinda citrifolia . J. Plant Biol. 51, 209–212 (2008). https://doi.org/10.1007/BF03030700
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DOI: https://doi.org/10.1007/BF03030700