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In vitro culture type and elicitation affects secoiridoid and xanthone LC–ESI–TOF MS profile and production in Centaurium erythraea

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Abstract

Centaurium erythraea is medicinal plant with high content of secoiridoid glycosides and xanthones with gastroprotective, hepatoprotective and antioxidative properties. In vitro culture based C. erythraea production would offer sustainable and economically feasible alternative to wild plant harvest provided that comparable productivity could be achieved in artificially propagated plants. To identify most productive C. erythraea system the secoiridoid glycoside and xanthone production and profiles using liquid chromatography-mass spectrometry were assessed in in vitro shoot, callus and cell suspension culture-derived material in comparison with wild C. erythraea plants. In vitro shoot culture was the most productive system for C. erythraea secoiridoid production (total yield 86 mg g−1 dw) and cell culture was the most productive system for phenolic compound production (19 mg GAE g−1 dw). The effect of elicitors yeast extract, methyljasmonate (MeJA) and chitosan on secondary metabolite yield indicated that elicitation with MeJA and chitosan significantly decreased yield of sweroside and eustomin, respectively. Significant quantitative differences of major secoiridoids and xanthones indicated that further methodological improvements for increasing yield of major secondary metabolites of in vitro origin need to be explored for in vitro cultivation to become a feasible alternative to C. erythraea wild plant harvest.

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Acknowledgments

The study was funded by the European Regional Development Fund project 2014/0038/2DP/2.1.1.1.0/14/APIA/VIAA/047 “Development of new cosmetic composition for skin renewal and whitening, using environmentally friendly technology”. Authors are grateful to the helpful anonymous reviewers.

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Authors and Affiliations

  1. Laboratory of Bioanalytical and Biodosimetry Methods, Faculty of Biology, University of Latvia, Ratsupites 7, Riga, 1068, Latvia

    Anete Boroduske, Martins Boroduskis & Nils Rostoks

  2. Faculty of Chemistry, University of Latvia, Jelgavas 1, Riga, 1004, Latvia

    Ilva Nakurte

  3. Botanical Garden, University of Latvia, Kandavas 2, Riga, 1083, Latvia

    Signe Tomsone & Madara Lazdane

Authors
  1. Anete Boroduske
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  2. Ilva Nakurte
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  3. Signe Tomsone
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  4. Madara Lazdane
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  5. Martins Boroduskis
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  6. Nils Rostoks
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Correspondence to Nils Rostoks.

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Boroduske, A., Nakurte, I., Tomsone, S. et al. In vitro culture type and elicitation affects secoiridoid and xanthone LC–ESI–TOF MS profile and production in Centaurium erythraea . Plant Cell Tiss Organ Cult 126, 567–571 (2016). https://doi.org/10.1007/s11240-016-1016-3

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  • DOI: https://doi.org/10.1007/s11240-016-1016-3

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