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Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses

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Abstract

The basic region/leucine zipper (bZIP) transcription factors are known to play key roles in response to abiotic stress. In this study, a bZIP gene (VqbZIP39) was isolated from grape (Vitis quinquangularis) and constitutively expressed in Arabidopsis under control of the cauliflower mosaic virus 35S promoter. The transgenic Arabidopsis thaliana plants showed enhance salt and drought stress tolerance during seed germination and in the seedling and mature plant stages. Various physiological parameters related to stress responses were analyzed to gain further insight into the role of VqbZIP39 and it was found that osmotic stress caused less damage to the transgenic seedlings than to the corresponding wild type plants. This correlated with an increase in endogenous ABA content as a consequence of the constitutive over-expression of VqbZIP39, and the up-regulated expression of stress-inducible target genes associated with tolerance of drought, high-salt, and oxidative stresses. Our results suggest that the expression of VqbZIP39 in A. thaliana likely enhances the tolerance to multiple abiotic stresses through the ABA signaling pathway, and may therefore have a similar function in the response to abiotic stresses in grape.

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Abbreviations

bZIP:

Basic region/leucine zipper

WT:

Wild type

CaMV:

Cauliflower mosaic virus

ORF:

Open reading frame

RT-PCR:

Reverse transcription-polymerase chain reaction

GFP:

Green fluorescent protein

GUS:

β-Glucuronidase

MS:

Murashige and Skoog

MDA:

Malondialdehyde

TCA:

Trichloroacetic acid

TBA:

Thiobarbituric acid

ELISA:

Enzyme-linked immunosorbent assay

NBT:

Nitro blue tetrazolium

DAB:

Diaminobenzidine

ROS:

Reactive oxygen species

MV:

Methylviologen

SOD:

Superoxide dismutase

CAT:

Catalase

POD:

Peroxidase

H2O2 :

Hydrogen peroxide

O2 − :

Superoxide anion

ABA:

Abscisic acid

cDNA:

Complementary deoxyribonucleic acid

FW:

Fresh weight

EDTA:

Ethylene diamine tetraacetic acid

NCED3:

9-Cis-epoxycarotenoid dioxygenase

RD:

Responsive to dehydration

KIN:

Protein kinase

ERD:

Early responsive to dehydration

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31272136), as well as the Program for Innovative Research Team of Grape Germplasm Resources and Breeding (2013KCT-25). We thank PlantScribe (www.plantscribe.com) for editing this manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Mingxing Tu, Xianhang Wang, Li Huang, Rongrong Guo & Xiping Wang

  2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Mingxing Tu, Xianhang Wang, Li Huang, Hongjing Zhang & Xiping Wang

  3. Research Institute of Grapes and Melon in Xinjiang Uygur Autonomous Region, Shanshan, 838200, Xinjiang, China

    Junshe Cai

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  1. Mingxing Tu
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Tu, M., Wang, X., Huang, L. et al. Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses. Plant Cell Tiss Organ Cult 125, 537–551 (2016). https://doi.org/10.1007/s11240-016-0969-6

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