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Influence of salicylic acid and methyl jasmonate elicitation on anthocyanin production in callus cultures of Rosa hybrida L.

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Abstract

This study was undertaken to investigate the effects of salicylic acid (SA) and methyl jasmonate (MeJA) on anthocyanin induction, biomass accumulation, and color value (CV) indices for both pigment content (PC) and pigment production (PP) in callus cultures of Rosa hybrida cv. Pusa Ajay. A concentration-dependent response was exhibited by cultures on SA and MeJA at different concentrations individually or in combinations to Euphorbia millii medium supplemented with 204.5 mM sucrose, 2.45 μM indole butyric acid and 2.33 μM kinetin. There was positive influence on both callus biomass and anthocyanin accumulation. Treatment with 0.5 μM MeJA was most effective in inducing anthocyanin biosynthesis in callus cultures. Anthocyanin accumulation in callus cultures was enhanced with the addition of SA and MeJA, but these did not differ significantly from control for the number of days required for pigment initiation and for color intensification. Moreover, the addition of 0.5 μM MeJA alone resulted in a higher frequency of color response (97.25 %), PC (3.48 ± 0.07 CV g−1 FW), and PP (1.56 ± 0.03 CV test tube−1) over control. In contrast, the presence of higher levels of SA (400 μM) and MeJA (5.0 μM) reduced frequency of color response, as well as levels of PC and PP. MeJA did not increase biomass accumulation but promoted frequency of color response, PC and PP. Hence, it was suggested that 0.5 μM MeJA promoted anthocyanin production in rose callus cultures. Significant correlation was found between frequency of response and each of the PC (r = 0.988) and PP (r = 0.990). Furthermore, PC and PP were also highly correlated (r = 0.998).

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Abbreviations

AdS:

Adenine sulphate

CRD:

Completely randomized design

CV:

Color value

EM:

Euphorbia millii medium

FW:

Fresh weight

HSD:

Honestly significant difference

IBA:

Indole-3-butyric acid

Kin:

Kinetin

MeJA:

Methyl jasmonate

MS:

Murashige and Skoog’s medium

PC:

Pigment content

PP:

Pigment production

SA:

Salicylic acid

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Acknowledgments

M.R. gratefully acknowledges the Indian Agricultural Research Institute, New Delhi for awarding Senior Research Fellowship during the course of this study.

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Authors and Affiliations

  1. Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Mohan Ram, K. V. Prasad, B. S. Hada & Surendra Kumar

  2. Division of Fruits and Horticultural Technology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    S. K. Singh

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  1. Mohan Ram
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  2. K. V. Prasad
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  3. S. K. Singh
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  4. B. S. Hada
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  5. Surendra Kumar
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Correspondence to Mohan Ram.

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Ram, M., Prasad, K.V., Singh, S.K. et al. Influence of salicylic acid and methyl jasmonate elicitation on anthocyanin production in callus cultures of Rosa hybrida L.. Plant Cell Tiss Organ Cult 113, 459–467 (2013). https://doi.org/10.1007/s11240-013-0287-1

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