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In vitro induction of polyploidy in Centella asiatica (L.) Urban

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Abstract

Mutational breeding was conducted using in vitro-grown shoot-tips of Centella asiatica (L.) Urban treated with colchicine (0.025–0.400% for 12–36 h) to induce polyploidy. Treated shoot-tips were grown on Murashige and Skoog (MS) medium supplemented with 4.54 μM thidiazuron (TDZ), and regenerated plantlets were acclimatized and transferred to soil. Two months following transfer to ex vitro conditions, ploidy levels of regenerated plants were determined by flow cytometry and by determining chromosome counts. Treating shoot-tips with colchicine concentrations ranging from 0.050–0.200% for 12–24 h promoted induction of tetraploids. Morphological and growth characteristics and the triterpenoid contents of the polyploids were also measured. Tetraploid plants demonstrated significantly longer stomata and a higher stomatal index compared to those of the diploid control plants. Furthermore, a positive trend in both biomass and triterpenoid production was obtained with the tetraploid and mixoploid plants of C. asiatica.

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Acknowledgment

Mr. Tanavat Kaensaksiri is grateful for financial support from the TRF Master Research Grants (MRG-WI515S098). This work is part of his master’s thesis.

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-ayudthaya Road, Bangkok, 10400, Thailand

    Tanavat Kaensaksiri & Sompop Prathanturarug

  2. Department of Plant Science, Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok, 10400, Thailand

    Puangpaka Soontornchainaksaeng

  3. Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-ayudthaya Road, Bangkok, 10400, Thailand

    Noppamas Soonthornchareonnon

Authors
  1. Tanavat Kaensaksiri
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  2. Puangpaka Soontornchainaksaeng
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  3. Noppamas Soonthornchareonnon
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  4. Sompop Prathanturarug
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Correspondence to Sompop Prathanturarug.

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Kaensaksiri, T., Soontornchainaksaeng, P., Soonthornchareonnon, N. et al. In vitro induction of polyploidy in Centella asiatica (L.) Urban. Plant Cell Tiss Organ Cult 107, 187–194 (2011). https://doi.org/10.1007/s11240-011-9969-8

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  • DOI: https://doi.org/10.1007/s11240-011-9969-8

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