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Establishment of efficient and rapid regeneration system for some diploid wild species of Arachis

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Abstract

Though peanut tissue culture has advanced to a considerable extent using a number of explants with the subsequent production of transgenic plants, wild Arachis species appeared to be recalcitrant to using similar explants. In this study, the use of cotyledonary nodes as explants prepared from 7-day old seedlings resulted in the development of a simple and rapid regeneration protocol for five diploid wild species including A. diogoi, A. stenosperma, A. duranensis, A. cardenasii and A. correntina belonging to the genus Arachis for producing multiple shoots. Shoot bud initiation was observed 10 to 15 days after culture initiation. Responding cotyledonary nodes with shoot buds were subcultured to lower levels of cytokinin and finally to MS basal medium for further shoot development and elongation. Production of multiple shoots was observed in all the five diploid species with a maximum of 9 to 16 shoots were obtained per explant in the primary cultures. The number of shoot buds increased significantly with repeated explant subculturing with recovery up to 45 shoots from responding explants. These shoots were rooted efficiently on MS medium supplemented with 1 mg l−1 naphthalene acetic acid and the time taken from explanting to the transfer of shoots to potting mixture was about 12 weeks. All rooted shoots were successfully established in soil in glass house and further transferred to field. These plants survived to maturity and set seed.

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Acknowledgments

The authors are thankful to the Volkswagen Foundation, Germany for supporting the work. Thanks are also due to International Crop Research Institute for the Semi-Arid tropics (ICRISAT), Patancheru, India for providing the germplasm of wild species of Arachis. TS is grateful to the University of Hyderabad for providing a Junior Research Fellowship under the “University with potential for Excellence” scheme and KRRK is grateful to the Council of Scientific and Industrial Research for a Junior Research Fellowship. Use of other Departmental facilities provided by DST-FIST, UGC-CAS etc. is gratefully acknowledged.

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  1. Tantravahi Srinivasan

    Present address: Department of Biotechnology, GITAM University, Visakhapatnam, 530045, India

Authors and Affiliations

  1. Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India

    Tantravahi Srinivasan, Koppolu Raja Rajesh Kumar & Pulugurtha Bharadwaja Kirti

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  1. Tantravahi Srinivasan
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  2. Koppolu Raja Rajesh Kumar
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  3. Pulugurtha Bharadwaja Kirti
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Correspondence to Tantravahi Srinivasan.

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Srinivasan, T., Kumar, K.R.R. & Kirti, P.B. Establishment of efficient and rapid regeneration system for some diploid wild species of Arachis . Plant Cell Tiss Organ Cult 101, 303–309 (2010). https://doi.org/10.1007/s11240-010-9689-5

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  • DOI: https://doi.org/10.1007/s11240-010-9689-5

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