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Direct somatic embryogenesis from axes of mature peanut embryos

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Summary

Plant regeneration via somatic embryogenesis was obtained in peanut (Arachis hypogaea L.) from axes of mature zygotic embryos. The area of greatest embryogenic activity was a 2-mm region adjacent to and encircling the epicotyl. Somatic embryogenesis was evaluated on Murashige and Skoog media supplemented with a variety of auxin treatments. Maximum production occurred on medium supplemented with 3 mg · liter−1 4-amino-3,5,6-trichloropicolinic acid. Explant cultures were transferred to half-strength medium supplemented with 1 mg · liter−1 gibberellic acid for somatic embryo germination and early plantlet growth. Plantlets, transferred to soil, were placed in a greenhouse and grown to maturity.

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  1. The Land, EPCOT Center, P.O. Box 10,000, 32830, Lake Buena Vista, Florida

    A. H. McKently

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  1. A. H. McKently
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McKently, A.H. Direct somatic embryogenesis from axes of mature peanut embryos. In Vitro Cell Dev Biol - Plant 27, 197–200 (1991). https://doi.org/10.1007/BF02632216

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  • DOI: https://doi.org/10.1007/BF02632216

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