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Regeneration of different cultivars of common bean (Phaseolus vulgaris L.) via indirect organogenesis

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Abstract

A protocol for in vitro regeneration via indirect organogenesis for Phaseolus vulgaris cv. Negro Jamapa was established. The explants used were apical meristems and cotyledonary nodes dissected from the embryonic axes of germinating seeds. Several auxin/cytokinin combinations were tested for callus induction. The best callus production was obtained with medium containing 1.5 μM 2,4-dichlorophenoxyacetic acid. After 2 weeks of growth calli were transferred to shooting medium containing 22.2 μM 6-benzylaminopurine. Shoots regenerated with a frequency of approximately 0.5 shoots per callus, and upon transfer to rooting medium these shoots produced roots with 100% efficiency. Histological analyses of the regeneration process confirmed the indirect organogenesis pattern. Greenhouse grown regenerated plants showed normal development and were fertile. The protocol was reproducible for other nine P. vulgaris cultivars tested, suggesting a genotype independent procedure.

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Abbreviations

AM:

Apical meristem

BAP:

6-Benzylaminopurine

CIM:

Callus induction medium

CN:

Cotyledonary node

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

MES:

2-(N-Morpholino) ethanesulfonic acid

MS:

Murashige and Skoog medium

NAA:

α-naphthaleneacetic acid

PVP-360:

Polyvinylpyrrolidone 360,000 molecular weight

SM:

Shooting medium

RM:

Rooting medium

References

  • Broughton WJ, Hernández G, Blair M, Beebe S, Gepts P, Vanderleyden J (2003) Beans (Phaseolus spp.)—model food legumes. Plant Soil 252:55–128. doi:10.1023/A:1024146710611

    Article  CAS  Google Scholar 

  • Chu CC, Wang CC, Sun SC, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–688

    Google Scholar 

  • Cruz de Carvalho MH, Van Le B, Zuily-Fodil Y, Pham Thi AT, Tran Thanh Van K (2000) Efficient whole plant regeneration of common bean (Phaseolus vulgaris L.) using thin-cell-layer culture and silver nitrate. Plant Sci 159:223–232. doi:10.1016/S0168-9452(00)00346-0

    Article  PubMed  CAS  Google Scholar 

  • Delgado-Sánchez P, Saucedo-Ruiz M, Guzmán-Maldonado SH, Villordo-Pineda E, González Chavira M, Fraire-Velázquez S, Acosta-Gallegos JA, Mora-Avilés A (2006) An organogenic plant regeneration system for common bean (Phaseolus vulgaris L). Plant Sci 170:822–827. doi:10.1016/j.plantsci.2005.11.015

    Article  Google Scholar 

  • Dillen W, De Clercq J, Goossens A, Van Montagu M, Angenon G (1997) Agrobacterium mediated transformation of Phaseolus acutifolius A. gray. Theor Appl Genet 94:151–158. doi:10.1007/s001220050394

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158. doi:10.1016/0014-4827(68)90403-5

    Article  PubMed  CAS  Google Scholar 

  • Malik KA, Saxena PK (1992) Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P.coccineus L., and P. wrightii L. Plant Cell Rep 11:163–168. doi:10.1007/BF00232172

    Article  CAS  Google Scholar 

  • Mohamed MF, Coyne DP, Read PE (1993) Shoot organogenesis in callus induced from pedicel explants of common bean (Phaseolus vulgaris L.). J Am Soc Hortic Sci 118:158–162

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Nagl W, Ignacimuthu S, Becker J (1997) Genetic engineering and regeneration of Phaseolus and Vigna. State of the art and new attempts. J Plant Physiol 150:625–644

    CAS  Google Scholar 

  • Pigeaire A, Abernethy D, Smith PM, Simpson K, Fletcher N, Lu CY, Atkins CA, Cornish E (1997) Transformation of a grain legume (Lupinus angustifolius L) via Agrobacterium tumefaciens-mediated gene transfer to shoot apices. Mol Breed 3:341–349. doi:10.1023/A:1009642620907

    Article  CAS  Google Scholar 

  • Rech EL, Giovanni R, Vianna GR, Aragao FJL (2008) High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants. Nat Protoc 3:410–418. doi:10.1038/nprot.2008.9

    Article  PubMed  CAS  Google Scholar 

  • Santalla M, Power JB, Davey MR (1998) Efficient in vitro shoot regeneration responses of Phaseolus vulgaris and P coccineus. Euphytica 102:195–202. doi:10.1023/A:1018317327302

    Article  Google Scholar 

  • Trepp GB, Van de Mortel M, Yoshioka H, Miller SS, Samac DA, Gant JS, Vance CP (1999) NADH-glutamate synthase in alfalfa root nodules. Genetic regulation and cellular expression. Plant Physiol 119:817–828. doi:10.1104/pp.119.3.817

    Article  PubMed  CAS  Google Scholar 

  • Zambre M, Goossens A, Cardona C, Van Montagu M, Terryn N, Angenon G (2005) A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil. Theor Appl Genet 110:914–924. doi:10.1007/s00122-004-1910-7

    Article  PubMed  CAS  Google Scholar 

  • Zambre MA, De Clercq J, Vranová E, Van Montagu M, Angenon G, Dillen W (1998) Plant regeneration from embryo-derived callus in Phaseolus vulgaris L (common bean) and P acutifolius A. Gray (tepary bean). Plant Cell Rep 17:626–630. doi:10.1007/s002990050455

    Article  CAS  Google Scholar 

  • Zambre MA, Geerts P, Maquet A, Van Montagu M, Dillen W, Angenon G (2001) Regeneration of fertile plants from callus in Phaseolus polyanthus Greenman (Year bean). Ann Bot (Lond) 88:371–377. doi:10.1006/anbo.2001.1468

    Article  CAS  Google Scholar 

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Acknowledgments

We thank Drs. M. Blair, S. Beebe and D. Debouck from CIAT for kindly supplying seeds, PhD Pallavolu Maheswara Reddy from CCG-UNAM for critical review, Victor Bustos from CCG-UNAM, Elizabeth González from CEIB-UAEM and to Ana Isabel Bieler Antolín from Microcine FC-UNAM, for technical assistance on plant maintenance, histological analysis and microphotography, respectively.

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Authors and Affiliations

  1. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Morelos, 62209, México

    Jesús Arellano, Sara Isabel Fuentes & Georgina Hernández

  2. Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca, Morelos, 62209, México

    Patricia Castillo-España

Authors
  1. Jesús Arellano
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  2. Sara Isabel Fuentes
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  3. Patricia Castillo-España
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  4. Georgina Hernández
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Correspondence to Georgina Hernández.

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Arellano, J., Fuentes, S.I., Castillo-España, P. et al. Regeneration of different cultivars of common bean (Phaseolus vulgaris L.) via indirect organogenesis. Plant Cell Tiss Organ Cult 96, 11–18 (2009). https://doi.org/10.1007/s11240-008-9454-1

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