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Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A. Gray (tepary bean)

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Abstract

Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius was obtained from mature embryo explants on a medium containing thidiazuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159, regeneration was achieved from cotyledon explants, but not from embryonic axis explants. Both explants could be used for the P. acutifolius genotype NI 574 but embryonic axes gave the best results. In-vitro-rooted plantlets of P. acutifolius could readily be established in the greenhouse. For P. vulgaris hardening problems with in-vitro-rooted plantlets could be overcome by means of in vitro grafting. The potential of the described procedure for obtaining transgenic P. vulgaris plants is discussed.

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Authors and Affiliations

  1. Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium Fax: +32-9-2645349 E-mail: mamon@gengenp.rug.ac.be, , , , , , BE

    M. A. Zambre, J. De Clercq, E. Vranová, M. Van Montagu, G. Angenon & W. Dillen

Authors
  1. M. A. Zambre
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  2. J. De Clercq
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  3. E. Vranová
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  4. M. Van Montagu
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  5. G. Angenon
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  6. W. Dillen
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Additional information

Received: 31 July 1997 / Revision received: 9 September 1997 / Accepted: 22 December 1997

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Zambre, M., De Clercq, J., Vranová, E. et al. Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A. Gray (tepary bean). Plant Cell Reports 17, 626–630 (1998). https://doi.org/10.1007/s002990050455

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  • DOI: https://doi.org/10.1007/s002990050455

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