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Micropropagation of Dendrobium densiflorum Lindl. ex Wall. through protocorm-like bodies: effects of plant growth regulators and lanthanoids

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Abstract

An efficient microprogation protocol has been developed for Dendrobium densiflorum Lindl. ex Wall., a traditional medicinal plant, through protocorm-like bodies (PLBs) from nodal stem segments using 6-benzylamino-purine (BAP) and the lanthanoid neodymium. The highest percentage of explants producing PLBs (72%), with an average of 15 PLBs per explant, was induced by culturing stem segments on Murashige and Skoog (MS) medium supplemented with 5.0 mg l−1 BAP. The newly formed PLBs proliferated well on the basal MS medium and completely converted into shoots on MS medium containing 2.0 mg l−1 BAP. Shoots produced an average of 22 roots per plantlet when cultured on MS medium supplemented with 2.0 mg l−1 neodymium nitrate. Healthy plantlets with well-developed roots were successfully acclimatized. The obtained result suggests that the lanthanoids can be used to effectively initiate rooting in the micropropagation and conservation of D. densiflorum.

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Abbreviations

AC:

Activated charcoal

BAP:

6-Benzylamino-purine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kin:

Kinetin

MS:

Murashige and Skoog (1962)

NAA:

α-Naphthaleneacetic acid

PLBs:

Protocorm-like bodies

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Acknowledgements

The supports from Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 20060359006) and the Research Fund for the Outstanding Doctor’s paper Program of Hefei University of Technology are gratefully acknowledged.

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Authors and Affiliations

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, The People’s Republic of China

    Jian-Ping Luo, Ying Wang, Xue-Qiang Zha & Li Huang

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  1. Jian-Ping Luo
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  2. Ying Wang
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  3. Xue-Qiang Zha
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  4. Li Huang
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Correspondence to Jian-Ping Luo.

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Luo, JP., Wang, Y., Zha, XQ. et al. Micropropagation of Dendrobium densiflorum Lindl. ex Wall. through protocorm-like bodies: effects of plant growth regulators and lanthanoids. Plant Cell Tiss Organ Cult 93, 333–340 (2008). https://doi.org/10.1007/s11240-008-9381-1

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